Anti-epidermal growth factor receptor siRNA carried by chitosan-transacylated lipid nanocapsules increases sensitivity of glioblastoma cells to temozolomide
| العنوان: | Anti-epidermal growth factor receptor siRNA carried by chitosan-transacylated lipid nanocapsules increases sensitivity of glioblastoma cells to temozolomide |
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| المؤلفون: | Frédéric Lagarce, Jean-Pierre Benoit, Khaled Messaoudi, Patrick Saulnier, Kim Boesen |
| المساهمون: | Micro et Nanomédecines Biomimétiques (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL), Département de Pharmacie [CHU d'Angers], Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Univ Angers, Okina |
| المصدر: | International Journal of Nanomedicine International Journal of Nanomedicine, Dove Medical Press, 2014, 9, pp.1479-90. ⟨10.2147/IJN.S59134⟩ International Journal of Nanomedicine, 2014, 9, pp.1479-90. ⟨10.2147/IJN.S59134⟩ |
| بيانات النشر: | Informa UK Limited, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Acylation, EGFR, [SDV]Life Sciences [q-bio], Cell, Biophysics, Pharmaceutical Science, Bioengineering, temozolomide, Biology, Pharmacology, Nanocapsules, Biomaterials, Transacylation, International Journal of Nanomedicine, Cell Line, Tumor, Drug Discovery, medicine, Humans, Gene Silencing, Epidermal growth factor receptor, Particle Size, RNA, Small Interfering, Cytotoxicity, Antineoplastic Agents, Alkylating, Original Research, Gene knockdown, lipid nanocapsules, Temozolomide, Organic Chemistry, glioblastoma, Drug Synergism, General Medicine, Transfection, Lipids, 3. Good health, Dacarbazine, ErbB Receptors, [SDV] Life Sciences [q-bio], Treatment Outcome, medicine.anatomical_structure, siRNA, biology.protein, chitosan, medicine.drug |
| الوصف: | Khaled Messaoudi,1 Patrick Saulnier,1 Kim Boesen,1 Jean-Pierre Benoit,1,2 Frederic Lagarce1,21L'Université Nantes Angers Le Mans, INSERM U1066, Micro et nanomédecines biomimétiques, Angers, France; 2Pharmacy Department, Angers University Hospital, Angers, FranceAbstract: Epidermal growth factor receptor (EGFR) is a crucial protein that plays an important role in the maintenance and development of glioblastomas. The silencing or knockdown of EGFR is possible by administering a small interfering ribonucleic acid (siRNA). Lipid nanocapsules (LNCs) covered by chitosan were developed in our laboratory by a transacylation process. The resulting nanocapsules have a positive zeta potential that enables electrostatic interactions with the negatively-charged siRNA. Prior to transfection, the cytotoxicity of the nanocapsules by (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) test was performed on the U87MG cell line to determine non-toxic levels of the LNCs to avoid cell mortality. Treatment of the U87MG cells with the chitosan-transacylated LNCs/anti-EGFR siRNA complex resulted in a reduction of EGFR expression by 51.95%±6.03% (P≤0.05) after 96 hours of incubation. It also increased the cellular sensitivity to temozolomide in comparison to untreated cells with siRNA. The largest increase in mortality was 62.55%±3.55% (P |
| وصف الملف: | text/html; application/pdf |
| تدمد: | 1178-2013 1176-9114 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10d700b213ea3fbef74e843600d6e713Test https://doi.org/10.2147/ijn.s59134Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....10d700b213ea3fbef74e843600d6e713 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 11782013 11769114 |
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