المؤلفون: Qi Chen, Fuchun Zhang, Qiao Teng, Lijie Xia

المصدر: International Journal of Nanomedicine. 15:2197-2205

مصطلحات موضوعية: Phage display, medicine.medical_treatment, Biophysics, Pharmaceutical Science, Bioengineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Flow cytometry, Targeted therapy, Biomaterials, Antigen, Affinity chromatography, In vivo, Drug Discovery, medicine, medicine.diagnostic_test, biology, Chemistry, Organic Chemistry, General Medicine, 021001 nanoscience & nanotechnology, Fusion protein, 0104 chemical sciences, Cancer research, biology.protein, Antibody, 0210 nano-technology

الوصف: Background Glypican-3 (GPC3) is a newly identified target molecule for the early diagnosis of hepatocellular carcinoma (HCC), while targeted inhibition of GPC3 signaling may help to control the proliferation and metastasis of HCC cells. The purpose of this study was to prepare the anti-GPC3 nanobody and to investigate the affinity of the anti-GPC3 nanobodies in vitro and the anticancer effects on hepatocellular carcinoma in vivo. Methods To screen for unknown anti-GPC3 antibodies, we constructed an antibody phage display library. After three rounds of panning, positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA). Further, the nanobody fusion protein was expressed in E. coli BL21 cells and purified by affinity chromatography. Competitive ELISA and flow cytometry were conducted to confirm the affinity of the anti-GPC3 nanobodies in vitro. The antitumor effects of VHHGPC3 were assessed in vivo. Results The results showed that the nanobody VHHGPC3 had specific high-affinity binding to His-GPC3 antigen. Moreover, VHHGPC3 exhibited specific binding to commercial human GPC3 and recognized the surface GPC3 protein of the hepatoma cell line HepG2. Importantly, in vivo study showed that GPC3 nanobody suppresses the growth of HepG2 and improves the survival rate of tumor mice. Discussion In summary, our new anti-GPC3 nanobody suggests a strong application potential for targeted therapy of liver cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::6efa529590d3999ad3335983ba5c4114Test
https://doi.org/10.2147/ijn.s235058Test

المؤلفون: Shi-Bin Wang, Ranjith Kumar Kankala, Ai-Zheng Chen, Chen-Guang Liu, Han-Xiao Tang, Biao-Qi Chen

المصدر: International Journal of Nanomedicine

مصطلحات موضوعية: Materials science, nanonization, Biophysics, Pharmaceutical Science, Biocompatible Materials, Bioengineering, Nanotechnology, Review, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biomaterials, Drug Delivery Systems, parameters effect, Drug Discovery, High surface area, chemistry.chemical_classification, Organic Chemistry, Green Chemistry Technology, General Medicine, Polymer, 021001 nanoscience & nanotechnology, Biodegradable polymer, Supercritical fluid, 0104 chemical sciences, Solutions, Pharmaceutical Preparations, chemistry, drug delivery, Drug delivery, anti-solvent, Pharmaceutical manufacturing, Particle, controlled release, 0210 nano-technology, Dispersion (chemistry), polymeric carriers

الوصف: In recent years, the supercritical fluid (SCF) technology has attracted enormous interest from researchers over the traditional pharmaceutical manufacturing strategies due to the environmentally benign nature and economically promising character of SCFs. Among all the SCF-assisted processes for particle formation, the solution-enhanced dispersion by supercritical fluids (SEDS) process is perhaps one of the most efficient methods to fabricate the biomaterials and pharmaceutical compounds at an arbitrary gauge, ranging from micro- to nanoscale. The resultant miniature-sized particles from the SEDS process offer enhanced features concerning their physical attributes such as bioavailability enhancement due to their high surface area. First, we provide a brief description of SCFs and their behavior as an anti-solvent in SCF-assisted processing. Then, we aim to give a brief overview of the SEDS process as well as its modified prototypes, highlighting the pros and cons of the particular modification. We then emphasize the effects of various processing constraints such as temperature, pressure, SCF as well as organic solvents (if used) and their flow rates, and the concentration of drug/polymer, among others, on particle formation with respect to the particle size distribution, precipitation yield, and morphologic attributes. Next, we aim to systematically discuss the application of the SEDS technique in producing therapeutic nano-sized formulations by operating the drugs alone or in combination with the biodegradable polymers for the application focusing oral, pulmonary, and transdermal as well as implantable delivery with a set of examples. We finally summarize with perspectives.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f677d50ef60ce599ef8294e6cbb8b0faTest
https://doi.org/10.2147/ijn.s166124Test

المؤلفون: Ding-Zhu Yang, Biao-Qi Chen, Kai Zhu, Shi-Bin Wang, Nina Jiang, Ranjith Kumar Kankala, Ai-Zheng Chen, Xiao-Xia Cheng

المصدر: International Journal of Nanomedicine

مصطلحات موضوعية: Biocompatibility, Cell Survival, Polyesters, Osteocalcin, Biophysics, Pharmaceutical Science, Fibroin, Bioengineering, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Collagen Type I, Cell Line, Biomaterials, Mice, Tissue engineering, Osteogenesis, International Journal of Nanomedicine, Drug Discovery, medicine, Cell Adhesion, Animals, Cell adhesion, Cell Proliferation, Original Research, Osteoblasts, Tissue Engineering, Tissue Scaffolds, Cell growth, Chemistry, Regeneration (biology), Organic Chemistry, cellular adhesion, Osteoblast, Cell Differentiation, General Medicine, surface topography, 021001 nanoscience & nanotechnology, Alkaline Phosphatase, 0104 chemical sciences, medicine.anatomical_structure, Alkaline phosphatase, Nanoparticles, Collagen, super critical fluids, bone engineering, 0210 nano-technology, Fibroins

الوصف: Biao-Qi Chen,1 Ranjith Kumar Kankala,1,2 Ai-Zheng Chen,1,2 Ding-Zhu Yang,1 Xiao-Xia Cheng,1 Ni-Na Jiang,1,2 Kai Zhu,3,4 Shi-Bin Wang1,2 1Institute of Biomaterials and Tissue Engineering, 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, 3Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 4Shanghai Institute of Cardiovascular Disease, Shanghai, People’s Republic of China Abstract: Attempts to reflect the physiology of organs is quite an intricacy during the tissue engineering process. An ideal scaffold and its surface topography can address and manipulate the cell behavior during the regeneration of targeted tissue, affecting the cell growth and differentiation significantly. Herein, silk fibroin (SF) nanoparticles were incorporated into poly(L-lactic acid) (PLLA) to prepare composite scaffolds via phase-inversion technique using supercritical carbon dioxide (SC-CO2). The SF nanoparticle core increased the surface roughness and hydrophilicity of the PLLA scaffolds, leading to a high affinity for albumin attachment. The in vitro cytotoxicity test of SF/PLLA scaffolds in L929 mouse fibroblast cells indicated good biocompatibility. Then, the in vitro interplay between mouse preosteoblast cell (MC3T3-E1) and various topological structures and biochemical cues were evaluated. The cell adhesion, proliferation, osteogenic differentiation and their relationship with the structures as well as SF content were explored. The SF/PLLA weight ratio (2:8) significantly affected the MC3T3-E1 cells by improving the expression of key players in the regulation of bone formation, ie, alkaline phosphatase (ALP), osteocalcin (OC) and collagen 1 (COL-1). These results suggest not only the importance of surface topography and biochemical cues but also the potential of applying SF/PLLA composite scaffolds as biomaterials in bone tissue engineering. Keywords: super critical fluids, surface topography, bone engineering, cellular adhesion, alkaline phosphatase

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الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96364ddafb245b1b7b29106b50f7d9eaTest
https://www.dovepress.com/investigation-of-silk-fibroin-nanoparticle-decorated-polyl-lactic-acid-peer-reviewed-article-IJNTest