Marker-Assisted Molecular Profiling, Deletion Mutant Analysis, and RNA-Seq Reveal a Disease Resistance Cluster Associated with Uromyces appendiculatus Infection in Common Bean Phaseolus vulgaris L
| العنوان: | Marker-Assisted Molecular Profiling, Deletion Mutant Analysis, and RNA-Seq Reveal a Disease Resistance Cluster Associated with Uromyces appendiculatus Infection in Common Bean Phaseolus vulgaris L |
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| المؤلفون: | Venkateswara R. Sripathi, Venu Kalavacharla, Marcial A. Pastor-Corrales, Antonette Todd, Phillip E. McClean, Nicole M. Donofrio, Rian K. Lee |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 18; Issue 6; Pages: 1109 |
| بيانات النشر: | MDPI AG, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.medical_specialty, Genotype, Virulence, Locus (genetics), Plant disease resistance, bean rust, Article, Catalysis, Inorganic Chemistry, Uromyces, 03 medical and health sciences, Molecular genetics, medicine, Cluster Analysis, Physical and Theoretical Chemistry, Molecular Biology, Gene, Spectroscopy, common bean, phaseolus, uromyces, RNA-seq, transcriptome, marker assisted profiling, delineation, Plant Diseases, Phaseolus, 2. Zero hunger, Genetics, biology, Basidiomycota, Organic Chemistry, food and beverages, General Medicine, biology.organism_classification, Computer Science Applications, 030104 developmental biology, Genetic marker |
| الوصف: | Common bean (Phaseolus vulgaris L.) is an important legume, useful for its high protein and dietary fiber. The fungal pathogen Uromyces appendiculatus (Pers.) Unger can cause major loss in susceptible varieties of the common bean. The Ur-3 locus provides race specific resistance to virulent strains or races of the bean rust pathogen along with Crg, (Complements resistance gene), which is required for Ur-3-mediated rust resistance. In this study, we inoculated two common bean genotypes (resistant “Sierra” and susceptible crg) with rust race 53 of U. appendiculatus, isolated leaf RNA at specific time points, and sequenced their transcriptomes. First, molecular markers were used to locate and identify a 250 kb deletion on chromosome 10 in mutant crg (which carries a deletion at the Crg locus). Next, we identified differential expression of several disease resistance genes between Mock Inoculated (MI) and Inoculated (I) samples of “Sierra” leaf RNA within the 250 kb delineated region. Both marker assisted molecular profiling and RNA-seq were used to identify possible transcriptomic locations of interest regarding the resistance in the common bean to race 53. Identification of differential expression among samples in disease resistance clusters in the bean genome may elucidate significant genes underlying rust resistance. Along with preserving favorable traits in the crop, the current research may also aid in global sustainability of food stocks necessary for many populations. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e12be0c7a47b85840a557747f195a06Test https://doi.org/10.3390/ijms18061109Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0e12be0c7a47b85840a557747f195a06 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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