دورية أكاديمية
HE4 Transcription- and Splice Variants-Specific Expression in Endometrial Cancer and Correlation with Patient Survival
| العنوان: | HE4 Transcription- and Splice Variants-Specific Expression in Endometrial Cancer and Correlation with Patient Survival |
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| المؤلفون: | Shi-Wen Jiang, Haibin Chen, Sean Dowdy, Alex Fu, John Attewell, Eleftheria Kalogera, Ronny Drapkin, Karl Podratz, Russell Broaddus, Jinping Li |
| المصدر: | International Journal of Molecular Sciences, Vol 14, Iss 11, Pp 22655-22677 (2013) |
| بيانات النشر: | MDPI AG, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | HE4, transcription, splice variants, gene expression, endometrial cancer, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | We investigated the HE4 variant-specific expression patterns in various normal tissues as well as in normal and malignant endometrial tissues. The relationships between mRNA variants and age, body weight, or survival are analyzed. ICAT-labeled normal and endometrial cancer (EC) tissues were analyzed with multidimensional liquid chromatography followed by tandem mass spectrometry. Levels of HE4 mRNA variants were measured by real-time PCR. Mean mRNA levels were compared among 16 normal endometrial samples, 14 grade 1 and 14 grade 3 endometrioid EC, 15 papillary serous EC, and 14 normal human tissue samples. The relationship between levels of HE4 variants and EC patient characteristics was analyzed with the use of Pearson correlation test. We found that, although all five HE4 mRNA variants are detectable in normal tissue samples, their expression is highly tissue-specific, with epididymis, trachea, breast and endometrium containing the highest levels. HE4-V0, -V1, and -V3 are the most abundant variants in both normal and malignant tissues. All variants are significantly increased in both endometrioid and papillary serous EC, with higher levels observed in grade 3 endometrioid EC. In the EC group, HE4-V1, -V3, and -V4 levels inversely correlate with EC patient survival, whereas HE4-V0 levels positively correlate with age. HE4 variants exhibit tissue-specific expression, suggesting that each variant may exert distinct functions in normal and malignant cells. HE4 levels appear to correlate with EC patient survival in a variant-specific manner. When using HE4 as a biomarker for EC management, the effects of age should be considered. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| العلاقة: | http://www.mdpi.com/1422-0067/14/11/22655Test; https://doaj.org/toc/1422-0067Test |
| DOI: | 10.3390/ijms141122655 |
| الوصول الحر: | https://doaj.org/article/28129aff1b8b4814a7ffdb5f0825f989Test |
| رقم الانضمام: | edsdoj.28129aff1b8b4814a7ffdb5f0825f989 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms141122655 |