المؤلفون: Eun-Soo Lee, Yujin Ahn, Il-Hong Bae, Daejin Min, Nok Hyun Park, Woonggyu Jung, Se-Hwa Kim, Yong Deog Hong, Won Seok Park, Chang Seok Lee

المصدر: International Journal of Molecular Sciences, Vol 21, Iss 9, p 3198 (2020)

مصطلحات موضوعية: seletinoid G, wound healing, keratinocyte, human skin equivalents, optical coherence tomography, second harmonic generation, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could be also considered as a potent function of an anti-aging reagent. Here, we examine the epidermal wound-healing efficacy of the fourth-generation retinoid, seletinoid G, using HaCaT keratinocytes and skin tissues. We found that seletinoid G promoted the proliferation and migration of keratinocytes in scratch assays and time-lapse imaging. It also increased the gene expression levels of several keratinocyte proliferation-regulating factors. In human skin equivalents, seletinoid G accelerated epidermal wound closure, as assessed using optical coherence tomography (OCT) imaging. Moreover, second harmonic generation (SHG) imaging revealed that seletinoid G recovered the reduced dermal collagen deposition seen in ultraviolet B (UVB)-irradiated human skin equivalents. Taken together, these results indicate that seletinoid G protects the skin barrier by accelerating wound healing in the epidermis and by repairing collagen deficiency in the dermis. Thus, seletinoid G could be a potent anti-aging agent for protecting the skin barrier.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/21/9/3198Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/390db2f394f045d78d14b37127208577Test

المؤلفون: Sung Hoon Lee, Il-Hong Bae, Eun-Soo Lee, Hyoung-June Kim, Jongsung Lee, Chang Seok Lee

المصدر: International Journal of Molecular Sciences, Vol 21, Iss 5, p 1736 (2020)

مصطلحات موضوعية: melanogenesis, sugar, glucose, tyrosinase, human skin equivalent, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Sugars are ubiquitous in organisms and well-known cosmetic ingredients for moisturizing skin with minimal side-effects. Glucose, a simple sugar used as an energy source by living cells, is often used in skin care products. Several reports have demonstrated that sugar and sugar-related compounds have anti-melanogenic effects on melanocytes. However, the underlying molecular mechanism by which glucose inhibits melanin synthesis is unknown, even though glucose is used as a whitening as well as moisturizing ingredient in cosmetics. Herein, we found that glucose significantly reduced the melanin content of α-melanocyte-stimulating hormone (MSH)-stimulated B16 cells and darkly pigmented normal human melanocytes with no signs of cytotoxicity. Furthermore, topical treatment of glucose clearly demonstrated its whitening efficacy through photography, Fontana-Masson (F&M) staining, and multi-photon microscopy in a pigmented 3D human skin model, MelanoDerm. However, glucose did not alter the gene expression or protein levels of major melanogenic proteins in melanocytes. While glucose potently decreased intracellular tyrosinase activity in melanocytes, it did not reduce mushroom tyrosinase activity in a cell-free experimental system. However, glucose was metabolized into lactic acid, which can powerfully suppress tyrosinase activity. Thus, we concluded that glucose indirectly inhibits tyrosinase activity through conversion into lactic acid, explaining its anti-melanogenic effects in melanocytes.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/21/5/1736Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/06263ea091404025b4156d0c77a97e11Test

المؤلفون: Su-Jin, Lim, Da-Won, Jung, Prima F, Hillman, Sang-Jip, Nam, Chang-Seok, Lee

المصدر: International journal of molecular sciences. 23(23)

مصطلحات موضوعية: Melanins, Microphthalmia-Associated Transcription Factor, Monophenol Monooxygenase, Plant Extracts, Cell Line, Tumor, Melanoma, Experimental, Animals, Cyclic AMP-Dependent Protein Kinases, Streptomyces

الوصف: Excess melanin in skin is known to be the main cause of hyper-pigmentary skin diseases such as freckles and lentigo. This study aimed to evaluate the depigmenting efficacy of an extract from the marine microorganism strain

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::87344be631f3ad2732b88e0667d933f2Test
https://pubmed.ncbi.nlm.nih.gov/36499251Test

المؤلفون: Il-Hong Bae, Eun-Soo Lee, Daejin Min, Se-Hwa Kim, Yong Deog Hong, Yujin Ahn, Woonggyu Jung, Nok Hyun Park, Chang Seok Lee, Won Seok Park

المصدر: International Journal of Molecular Sciences
Volume 21
Issue 9
International Journal of Molecular Sciences, Vol 21, Iss 3198, p 3198 (2020)

مصطلحات موضوعية: Keratinocytes, 0301 basic medicine, Human skin, wound healing, lcsh:Chemistry, 030207 dermatology & venereal diseases, 0302 clinical medicine, Cell Movement, Retinoid, lcsh:QH301-705.5, Spectroscopy, Barrier function, Skin, integumentary system, Chemistry, Dioxolanes, General Medicine, Computer Science Applications, Cell biology, medicine.anatomical_structure, Keratinocyte, Tomography, Optical Coherence, seletinoid G, Cell Survival, Ultraviolet Rays, medicine.drug_class, keratinocyte, Article, Catalysis, Cell Line, Inorganic Chemistry, 03 medical and health sciences, Dermis, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, Pyrans, optical coherence tomography, second harmonic generation, Organic Chemistry, HaCaT, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, human skin equivalents, Epidermis, Wound healing

الوصف: The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could be also considered as a potent function of an anti-aging reagent. Here, we examine the epidermal wound-healing efficacy of the fourth-generation retinoid, seletinoid G, using HaCaT keratinocytes and skin tissues. We found that seletinoid G promoted the proliferation and migration of keratinocytes in scratch assays and time-lapse imaging. It also increased the gene expression levels of several keratinocyte proliferation-regulating factors. In human skin equivalents, seletinoid G accelerated epidermal wound closure, as assessed using optical coherence tomography (OCT) imaging. Moreover, second harmonic generation (SHG) imaging revealed that seletinoid G recovered the reduced dermal collagen deposition seen in ultraviolet B (UVB)-irradiated human skin equivalents. Taken together, these results indicate that seletinoid G protects the skin barrier by accelerating wound healing in the epidermis and by repairing collagen deficiency in the dermis. Thus, seletinoid G could be a potent anti-aging agent for protecting the skin barrier.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4187cf7d887e9ee8f5ef57b797e818cTest

المؤلفون: Minjeong Kim, Chang Seok Lee, Kyung Min Lim

المصدر: International Journal of Molecular Sciences
Volume 20
Issue 22

مصطلحات موضوعية: 0301 basic medicine, Leukoderma, Butanols, Cell, rhododenol, Vitiligo, Human skin, chemical and pharmacologic phenomena, Biology, Melanocyte, Models, Biological, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Mice, 0302 clinical medicine, Cell Line, Tumor, morphology, medicine, Cytotoxic T cell, leukoderma, Animals, Humans, Trypsin, Physical and Theoretical Chemistry, Cytoskeleton, Molecular Biology, Spectroscopy, Melanosome, integumentary system, Dose-Response Relationship, Drug, Monophenol Monooxygenase, Organic Chemistry, cytoskeleton, General Medicine, medicine.disease, Computer Science Applications, Cell biology, melanocytes, Cytoskeletal Proteins, 030104 developmental biology, medicine.anatomical_structure, Gene Expression Regulation, 030220 oncology & carcinogenesis, Melanoderm™

الوصف: Rhododenol (RD), a whitening cosmetic ingredient, was withdrawn from the market due to RD-induced leukoderma (RIL). While many attempts have been made to clarify the mechanism underlying RIL, RIL has not been fully understood yet. Indeed, affected subjects showed uneven skin pigmentation, but the features are different from vitiligo, a skin hypopigmentary disorder, alluding to events more complex than simple melanocyte cytotoxicity. Here, we discovered that rhododenol treatment reduced the number of melanocytes in a pigmented 3D human skin model, Melanoderm&trade
confirming the melanocyte toxicity of RD. Of note, melanocytes that survived in the RD treated tissues exhibited altered morphology, such as extended dendrites and increased cell sizes. Consistently with this, sub-cytotoxic level of RD increased cell size and elongated dendrites in B16 melanoma cells. Morphological changes of B16 cells were further confirmed in the immunocytochemistry of treated cells for actin and tubulin. Even more provoking, RD up-regulated the expression of tyrosinase and TRP1 in the survived B16 cells. Evaluation of mRNA expression of cytoskeletal proteins suggests that RD altered the cytoskeletal dynamic favoring cell size expansion and melanosome maturation. Collectively, these results suggest that RD not only induces cytotoxicity in melanocytes but also can lead to a profound perturbation of melanocyte integrity even at sub-cytotoxic levels.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::301ed88a67444150bf20889465a7e179Test
http://europepmc.org/articles/PMC6888388Test