Dose- and Time-Dependent Effects of Oleate on Mitochondrial Fusion/Fission Proteins and Cell Viability in HepG2 Cells: Comparison with Palmitate Effects
| العنوان: | Dose- and Time-Dependent Effects of Oleate on Mitochondrial Fusion/Fission Proteins and Cell Viability in HepG2 Cells: Comparison with Palmitate Effects |
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| المؤلفون: | Gaetana Paolella, Lillà Lionetti, Ivana Caputo, Isy Faria de Sousa, Vincenzo Migliaccio, Eliane Beraldi Ribeiro, Marilena Lepretti, Ilaria Di Gregorio |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 9812, p 9812 (2021) Volume 22 Issue 18 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Dynamins, QH301-705.5, Cell Survival, MFN2, Palmitates, DRP1, Mitochondrion, Mitochondrial Dynamics, Catalysis, Article, GTP Phosphohydrolases, Inorganic Chemistry, Fatty Acids, Monounsaturated, Mitochondrial Proteins, Mitofusin-2, Metabolic Diseases, Humans, saturated fatty acids, Viability assay, Physical and Theoretical Chemistry, Biology (General), Molecular Biology, QD1-999, Spectroscopy, Dose-Response Relationship, Drug, Chemistry, Organic Chemistry, monounsaturated fatty acids, General Medicine, Cellular adaptation, Hepatocytes, Lipotoxicity, Mitochondrial dynamics, Monounsaturated fatty acids, Saturated fatty acids, Hep G2 Cells, lipotoxicity, Lipid Metabolism, Lipids, Computer Science Applications, Cell biology, Mitochondria, cellular adaptation, mitochondrial fusion, Gene Expression Regulation, Saturated fatty acid, Hepatic stellate cell, hepatocytes, Oleic Acid |
| الوصف: | Mitochondrial impairments in dynamic behavior (fusion/fission balance) associated with mitochondrial dysfunction play a key role in cell lipotoxicity and lipid-induced metabolic diseases. The present work aimed to evaluate dose- and time-dependent effects of the monounsaturated fatty acid oleate on mitochondrial fusion/fission proteins in comparison with the saturated fatty acid palmitate in hepatic cells. To this end, HepG-2 cells were treated with 0, 10 μM, 50 μM, 100 μM, 250 μM or 500 μM of either oleate or palmitate for 8 or 24 h. Cell viability and lipid accumulation were evaluated to assess lipotoxicity. Mitochondrial markers of fusion (mitofusin 2, MFN2) and fission (dynamin-related protein 1, DRP1) processes were evaluated by Western blot analysis. After 8 h, the highest dose of oleate induced a decrease in DRP1 content without changes in MFN2 content in association with cell viability maintenance, whereas palmitate induced a decrease in cell viability associated with a decrease mainly in MFN2 content. After 24 h, oleate induced MFN2 increase, whereas palmitate induced DRP1 increase associated with a higher decrease in cell viability with high doses compared to oleate. This finding could be useful to understand the role of mitochondria in the protective effects of oleate as a bioactive compound. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::857f7848385afc75a6e7019d481a36abTest https://pubmed.ncbi.nlm.nih.gov/34575980Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....857f7848385afc75a6e7019d481a36ab |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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