المؤلفون: Gil-Kulik, Paulina, Leśniewski, Michał, Bieńko, Karolina, Wójcik, Monika, Więckowska, Marta, Przywara, Dominika, Petniak, Alicja, Kondracka, Adrianna, Świstowska, Małgorzata, Szymanowski, Rafał, Wilińska, Agnieszka, Wiliński, Mateusz, Płachno, Bartosz J., Kostuch, Marzena, Rahnama-Hezavach, Mansur, Szuta, Mariusz, Kwaśniewska, Anna, Bogucka-Kocka, Anna, Kocki, Janusz

المصدر: International Journal of Molecular Sciences; Feb2023, Vol. 24 Issue 3, p2476, 20p

مصطلحات موضوعية: GENE expression, PERINATAL death, BREAST milk, STEM cells, MATERNAL age, MESENCHYMAL stem cells, PREMATURE labor, CHILDBIRTH, IMMUNOLOGIC diseases

مستخلص: Due to their therapeutic potential, mesenchymal stem cells are the subject of intensive research on the use of their potential in the treatment of, among others, neurodegenerative diseases or immunological diseases. They are among the newest in the field of medicine. The presented study aimed to evaluate the expression of eight genes from the IAP family and the gene regulating IAP—XAF1—in stem cells derived from human milk, using the qPCR method. The relationships between the expression of genes under study and clinical data, such as maternal age, maternal BMI, week of pregnancy in which the delivery took place, bodyweight of the newborn, the number of pregnancies and deliveries, and the time elapsed since delivery, were also analyzed. The research was carried out on samples of human milk collected from 42 patients hospitalized in The Clinic of Obstetrics and Perinatology of the Independent Public Clinical Hospital No. 4, in Lublin. The conducted research confirmed the expression of the following genes in the tested material: NAIP, BIRC2, BIRC3, BIRC5, BIRC6, BIRC8, XIAP, XAF1, OCT4 and SOX2. Moreover, several dependencies of the expression of individual genes on the maternal BMI (BIRC5, XAF1 and NAIP), the time since childbirth (BIRC5, BIRC6, XAF1 and NAIP), the number of pregnancies and deliveries (BIRC2, BIRC5, BIRC6 and XAF1), the manner of delivery (XAF1 and OCT4), preterm labor (BIRC6 and NAIP) were demonstrated. Additionally, we found positive relationships between gene expression of BIRC7, BIRC8 and XAF1 and the main factors of pluripotency: SOX2 and OCT4. This work is the first to investigate the expression of genes from the IAPs family in mother's milk stem cells. [ABSTRACT FROM AUTHOR]

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المؤلفون: Gródecka-Szwajkiewicz, Dorota¹ (AUTHOR) kawamilosz@gmail.com, Ulańczyk, Zofia¹ (AUTHOR), Zagrodnik, Edyta¹ (AUTHOR), Łuczkowska, Karolina¹ (AUTHOR), Rogińska, Dorota¹ (AUTHOR), Kawa, Miłosz P.¹ (AUTHOR), Stecewicz, Iwona¹ (AUTHOR), Safranow, Krzysztof² (AUTHOR), Machaliński, Bogusław¹ (AUTHOR) machalin@pum.edu.pl

المصدر: International Journal of Molecular Sciences. Feb2020, Vol. 21 Issue 4, p1305. 1p.

مصطلحات موضوعية: *CORD blood, *PREMATURE labor, *SECRETION, *CHILDBIRTH, *MICRORNA, *PREMATURE infants

مستخلص: Objectives: Premature birth, defined as less than 37 weeks gestation, affects approximately 12% of all live births around the world. Advances in neonatal care have resulted in the increased survival of infants born prematurely. Although prematurity is a known risk factor for different cardiovascular diseases, little is known about the pathophysiology of vasculature during premature gestation and angiopoietic factors network during premature birth. Aims: The objective of this study was to determine whether the profile of several pro-angiogenic and anti-angiogenic factors in umbilical cord blood (UCB) is different in healthy appropriate-for-gestational-age preterm newborns and normal term babies. The second aim of this study was to investigate the microRNA (miRNAs) expression profile in UCB from preterm labor and to detect miRNAs potentially taking part in control of angogenesis-related processes (Angio-MiRs). Methods: Using an immunobead Luminex assay, we simultaneously measured the concentration of Angiogenin, Angiopoietin-1, FGF-acidic, FGF-basic, PDGF-aa, PlGF, VEGF, VEGF-D, Endostatin, Thrombospondin-2, NGF, BDNF, GDNF, and NT-4 in UCB samples collected from the preterm (n = 27) and term (n = 52) delivery. In addition, the global microRNA expression in peripheral blood mononuclear cells (PBMCs) circulating in such UCB samples was examined in this study using microarray MiRNA technique. Results: The concentrations of five from eight measured pro-angiogenic factors (VEGF, Angiopoietin-1, PDGF-AA, FGF-a, and FGF-b) were significantly lower in UCB from preterm newborns. On the contrary, two angiostatic factors (Endostatin and Thrombospondin-2) were significantly up-regulated in preterm UCB. Among analyzed neurotrophins in preterm newborns, the elevated UCB concentration was found only in the case of GDNF, whereas BDNF was significantly reduced. Moreover, two angiopoietic factors, VEGF-D and PlGF, and two neurotrophins, NT4 and NGF, did not differ in concentration in preterm and term babies. We also discovered that among the significantly down-regulated miRNAs, there were several classical Angio-MiRs (inter alia MiR-125, MiR-126, MiR-145, MiR-150, or MiR155), which are involved in angiogenesis regulation in newborn after preterm delivery. Conclusions: This is the first report of simultaneous measurements of several angiopoietic factors in UCB collected from infants during preterm and term labor. Here, we observed that several pro-angiogenic factors were at lower concentration in UCB collected from preterm newborns than term babies. In contrast, the two measured angiostatic factors, Endostatin and Thrombospondin-2, were significantly higher in UCB from preterm babies. This can suggest that distinct pathophysiological contributions from differentially expressed various angiopoietic factors may determine the clinical outcomes after preterm birth. Especially, our angiogenesis-related molecules analysis indicates that preterm birth of healthy, appropriate-for-gestational-age newborns is an "anti-angiogenic state" that may provide an increased risk for improper development and function of cardiovascular system in the adulthood. This work also contributes to a better understanding of the role of miRNAs potentially involved in angiogenesis control in preterm newborns. [ABSTRACT FROM AUTHOR]