Engineered Mesenchymal Stem Cells Expressing Stromal Cell-derived Factor-1 Improve Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats
| العنوان: | Engineered Mesenchymal Stem Cells Expressing Stromal Cell-derived Factor-1 Improve Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats |
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| المؤلفون: | Hyun Cheol Jeong, Sae Woong Choi, Woong Jin Bae, Hyuk Jin Cho, Hyo-Jin Kim, Soon Min Lee, H. S. Kim, U-Syn Ha, Guan Qun Zhu, Sae Woong Kim, Seung Hwan Jeon, Ji Youl Lee, Sung-Hoo Hong, Eunbi Kwon |
| المصدر: | International Journal of Molecular Sciences, Vol 19, Iss 12, p 3730 (2018) International Journal of Molecular Sciences Volume 19 Issue 12 |
| بيانات النشر: | MDPI AG, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Blood Glucose, Male, endocrine system diseases, Basic fibroblast growth factor, Gene Expression, Nitric Oxide Synthase Type I, lcsh:Chemistry, chemistry.chemical_compound, Phosphatidylinositol 3-Kinases, Cell Movement, Medicine, Stromal cell-derived factor 1, stromal cell-derived factor-1 expressing engineered mesenchymal stem cells, lcsh:QH301-705.5, Spectroscopy, biology, General Medicine, Computer Science Applications, Vascular endothelial growth factor, Genetic Engineering, medicine.drug, Signal Transduction, medicine.medical_specialty, streptozotocin-induced diabetic rats, Stromal cell, intracavernosal injection, Nitric Oxide Synthase Type III, erectile dysfunction, Neovascularization, Physiologic, Endothelial NOS, Mesenchymal Stem Cell Transplantation, Catalysis, Article, Diabetes Mellitus, Experimental, Inorganic Chemistry, 03 medical and health sciences, Internal medicine, Diabetes mellitus, Animals, Physical and Theoretical Chemistry, Molecular Biology, Protein kinase B, business.industry, Organic Chemistry, Body Weight, nutritional and metabolic diseases, Mesenchymal Stem Cells, Streptozotocin, medicine.disease, Chemokine CXCL12, Rats, Disease Models, Animal, 030104 developmental biology, Endocrinology, chemistry, lcsh:Biology (General), lcsh:QD1-999, biology.protein, business, Proto-Oncogene Proteins c-akt |
| الوصف: | Effective therapies for erectile dysfunction (ED) associated with diabetes mellitus (DM) are needed. In this study, the effects of stromal cell-derived factor-1 (SDF-1)-expressing engineered mesenchymal stem cells (SDF-1 eMSCs) and the relevant mechanisms in the corpus cavernosum of a streptozotocin (STZ)-induced DM ED rat model were evaluated. In a randomized controlled trial, Sprague&ndash Dawley (SD) rats (n = 48) were divided into four groups (n = 12/group): Normal (control), DM ED (diabetes induced by STZ), DM ED + BM-MSC (treated with bone marrow [BM]-derived MSCs), and DM ED + SDF-1 eMSC (treated with SDF-1-expressing BM-MSCs). After four weeks, intracavernosal pressure (ICP), an indicator of erectile function, was 0.75 ± 0.07 in the normal group, 0.27 ± 0.08 in the DM ED group, 0.42 ± 0.11 in the DM ED + BM-MSC group, and 0.58 ± 0.11 in the DM ED + SDF-1 eMSC group. BM-MSCs, especially SDF-1 eMSCs, improved ED (p < 0.05). SDF-1 eMSC treatment improved the smooth muscle content in the corpus cavernosum (p < 0.05). As SDF-1 expression increased, ED recovery improved. In the SDF-1 eMSC group, levels of neuronal nitric oxide synthase (nNOS) and phosphorylated endothelial NOS (p-eNOS) were higher than those in other groups (p < 0.05). In addition, high stromal cell-derived factor-1 (SDF-1) expression was associated with increased vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in DM ED rats (p < 0.05). Higher levels of phosphorylated protein kinase B (p-AKT)/protein kinase B (AKT) (p < 0.05) and B-cell lymphoma-2 (Bcl-2) and lower levels of the apoptosis factors Bcl2-associated x (Bax) and caspase-3 were observed in the MSC-treated group than in the DM ED group (p < 0.05). SDF-1 eMSCs showed beneficial effects on recovery from erectile function. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e9b24305fc9392212ec07d6ebceb5a2Test https://www.mdpi.com/1422-0067/19/12/3730Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8e9b24305fc9392212ec07d6ebceb5a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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