Integrated Transcriptional and Proteomic Profiling Reveals Potential Amino Acid Transporters Targeted by Nitrogen Limitation Adaptation
| العنوان: | Integrated Transcriptional and Proteomic Profiling Reveals Potential Amino Acid Transporters Targeted by Nitrogen Limitation Adaptation |
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| المؤلفون: | Tian-Jiao Tang, Ting Zhou, Qiong Liao, Haixing Song, Zhenhua Zhang, Ying-Peng Hua |
| المصدر: | International Journal of Molecular Sciences Volume 21 Issue 6 International Journal of Molecular Sciences, Vol 21, Iss 6, p 2171 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Chlorophyll, Proteomics, 0106 biological sciences, 0301 basic medicine, Aging, Amino Acid Transport Systems, Mutant, Arabidopsis, 01 natural sciences, amino acid transporter, Anthocyanins, Transcriptome, lcsh:Chemistry, chemistry.chemical_compound, N limitation, Gene Expression Regulation, Plant, Tandem Mass Spectrometry, Protein biosynthesis, Photosynthesis, lcsh:QH301-705.5, Cellular Senescence, Spectroscopy, transcriptional profiling, chemistry.chemical_classification, N remobilization, medicine.diagnostic_test, food and beverages, General Medicine, proteomic change, Adaptation, Physiological, Computer Science Applications, Amino acid, Biochemistry, NLA, Nitrogen, Ubiquitin-Protein Ligases, Proteolysis, Article, Catalysis, Inorganic Chemistry, 03 medical and health sciences, medicine, Amino acid transporter, Physical and Theoretical Chemistry, Molecular Biology, Arabidopsis Proteins, Proteomic Profiling, Gene Expression Profiling, Organic Chemistry, fungi, Plant Leaves, Gene Ontology, 030104 developmental biology, chemistry, lcsh:Biology (General), lcsh:QD1-999, Protein Biosynthesis, Amino Acid Transport Systems, Basic, Chromatography, Liquid, 010606 plant biology & botany |
| الوصف: | Nitrogen (N) is essential for plant growth and crop productivity. Organic N is a major form of remobilized N in plants&rsquo response to N limitation. It is necessary to understand the regulatory role of N limitation adaption (NLA) in organic N remobilization for this adaptive response. Transcriptional and proteomic analyses were integrated to investigate differential responses of wild-type (WT) and nla mutant plants to N limitation and to identify the core organic N transporters targeted by NLA. Under N limitation, the nla mutant presented an early senescence with faster chlorophyll loss and less anthocyanin accumulation than the WT, and more N was transported out of the aging leaves in the form of amino acids. High-throughput transcriptomic and proteomic analyses revealed that N limitation repressed genes involved in photosynthesis and protein synthesis, and promoted proteolysis these changes were higher in the nla mutant than in the WT. Both transcriptional and proteomic profiling demonstrated that LHT1, responsible for amino acid remobilization, were only significantly upregulated in the nla mutant under N limitation. These findings indicate that NLA might target LHT1 and regulate organic N remobilization, thereby improving our understanding of the regulatory role of NLA on N remobilization under N limitation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms21062171 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba2bbe518e427e6b38ed9aad5d5af295Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ba2bbe518e427e6b38ed9aad5d5af295 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms21062171 |