A New Criterion to Evaluate Water Vapor Interference in Protein Secondary Structural Analysis by FTIR Spectroscopy
| العنوان: | A New Criterion to Evaluate Water Vapor Interference in Protein Secondary Structural Analysis by FTIR Spectroscopy |
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| المؤلفون: | Gang Ma, Ye Zou |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 15, Iss 6, Pp 10018-10033 (2014) Volume 15 Issue 6 Pages: 10018-10033 |
| بيانات النشر: | MDPI, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Absorption spectroscopy, Infrared, Analytical chemistry, Infrared spectroscopy, Interference (wave propagation), Fourier self-deconvolution, Catalysis, Article, Protein Structure, Secondary, Inorganic Chemistry, lcsh:Chemistry, symbols.namesake, Spectroscopy, Fourier Transform Infrared, Animals, Physical and Theoretical Chemistry, Fourier transform infrared spectroscopy, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, Second derivative, Chemistry, Organic Chemistry, Reproducibility of Results, General Medicine, vibrational spectroscopy, second derivative, Computer Science Applications, FTIR spectroscopy, Steam, Fourier transform, lcsh:Biology (General), lcsh:QD1-999, symbols, Muramidase, protein secondary structure, Chickens, Water vapor, Algorithms |
| الوصف: | Second derivative and Fourier self-deconvolution (FSD) are two commonly used techniques to resolve the overlapped component peaks from the often featureless amide I band in Fourier transform infrared (FTIR) curve-fitting approach for protein secondary structural analysis. Yet, the reliability of these two techniques is greatly affected by the omnipresent water vapor in the atmosphere. Several criteria are currently in use as quality controls to ensure the protein absorption spectrum is negligibly affected by water vapor interference. In this study, through a second derivative study of liquid water, we first argue that the previously established criteria cannot guarantee a reliable evaluation of water vapor interference due to a phenomenon that we refer to as sample’s absorbance-dependent water vapor interference. Then, through a comparative study of protein and liquid water, we show that a protein absorption spectrum can still be significantly affected by water vapor interference even though it satisfies the established criteria. At last, we propose to use the comparison between the second derivative spectra of protein and liquid water as a new criterion to better evaluate water vapor interference for more reliable second derivative and FSD treatments on the protein amide I band. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0b5f39e639a46227fa9483bdc7bba85Test http://europepmc.org/articles/PMC4100137Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b0b5f39e639a46227fa9483bdc7bba85 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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