Heat- and 4-hydroperoxy-ifosfamide-induced apoptosis in B cell precursor leukaemias
| العنوان: | Heat- and 4-hydroperoxy-ifosfamide-induced apoptosis in B cell precursor leukaemias |
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| المؤلفون: | U Banning, Dietzsch S, Ralf-Bodo Tröbs, Christine Mauz-Körholz, Dieter Körholz |
| المصدر: | International Journal of Hyperthermia. 19:444-460 |
| بيانات النشر: | Informa UK Limited, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Hyperthermia, Cancer Research, Programmed cell death, Hot Temperature, Physiology, medicine.medical_treatment, Antineoplastic Agents, Apoptosis, chemistry.chemical_compound, Cell Line, Tumor, Physiology (medical), Acute lymphocytic leukemia, Leukemia, B-Cell, medicine, Humans, Ifosfamide, B cell, Chemotherapy, business.industry, Intracellular Membranes, medicine.disease, Nitrogen mustard, medicine.anatomical_structure, chemistry, Caspases, Immunology, Cancer research, business, Precancerous Conditions, medicine.drug |
| الوصف: | In the group of high risk childhood acute lymphoblastic leukaemia (ALL), very early and early relapses have a very poor prognosis with conventional chemotherapy alone. Remission induction in these patients is often hindered by drug resistance. Thus, intensifying chemotherapy strategies are required. Application of hyperthermia enhances efficacy of certain anti-neoplastic drugs such as ifosfamide. In this study, effects and molecular mechanisms of ifosfamide - and hyperthermia-induced apoptosis are investigated in a B cell precursor leukaemia cell line (REH) and in primary patient-derived B cell progenitor leukaemic blasts. Both 4OOH-IFA and hyperthermia are able to induce cell death in leukaemic cells, mainly by induction of caspase-dependent apoptosis. However, completely different kinetics of caspase-3, -8 and -9 activation are found for both stimuli. In addition, activation of caspase-1 is only observed following stimulation with hyperthermia. Combined application of ifosfamide and hyperthermia reveals increased cytotoxicity in both the leukaemia cell line and in 5/8 of the patient-derived leukaemic blast samples. In conclusion, hyperthermia and ifosfamide mediate cytotoxicity in B precursor leukaemic blasts by different kinetics of caspase activation. This might explain the additive effects of 4OOH-IFA and heat on leukaemic cell death. Therefore, whole body thermochemotherapy could be considered as a treatment option in relapsed leukaemic patients. |
| تدمد: | 1464-5157 0265-6736 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bab2bc6bd7238f100431aa3cd9f88d7bTest https://doi.org/10.1080/0265673031000063864Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bab2bc6bd7238f100431aa3cd9f88d7b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14645157 02656736 |
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