In Vitro Consequences of Electronic-Cigarette Flavoring Exposure on the Immature Lung
| العنوان: | In Vitro Consequences of Electronic-Cigarette Flavoring Exposure on the Immature Lung |
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| المؤلفون: | Satyan Lakshminrusimha, Sara K. Berkelhamer, Maciej L. Goniewicz, Sylvia F. Gugino, Noel J. Leigh, Justin Helman |
| المصدر: | International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 16, Iss 19, p 3635 (2019) International journal of environmental research and public health, vol 16, iss 19 Volume 16 Issue 19 |
| بيانات النشر: | MDPI, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Health, Toxicology and Mutagenesis, lcsh:Medicine, Pharmacology, Electronic Nicotine Delivery Systems, Toxicology, Fetal Development, chemistry.chemical_compound, 0302 clinical medicine, Smooth Muscle, 2.2 Factors relating to the physical environment, immature lung, 030212 general & internal medicine, Aetiology, Lung, Pediatric, 0303 health sciences, Chemistry, food and beverages, electronic cigarette flavorings, Menthol, medicine.anatomical_structure, electronic cigarettes, Toxicity, Respiratory, Programmed cell death, Myocytes, Smooth Muscle, In Vitro Techniques, Pulmonary Artery, Article, 03 medical and health sciences, Lactate dehydrogenase, medicine.artery, Tobacco, Toxicity Tests, medicine, Animals, 030304 developmental biology, lung development, Fetus, Myocytes, Sheep, Tobacco Smoke and Health, lcsh:R, Public Health, Environmental and Occupational Health, toxicity, Newborn, In vitro, Flavoring Agents, Good Health and Well Being, Animals, Newborn, Pulmonary artery |
| الوصف: | Background: The developing lung is uniquely susceptible and may be at increased risk of injury with exposure to e-cigarette constituents. We hypothesize that cellular toxicity and airway and vascular responses with exposure to flavored refill solutions may be altered in the immature lung. Methods: Fetal, neonatal, and adult ovine pulmonary artery smooth muscle cells (PASMC) were exposed to popular flavored nicotine-free e-cigarette refill solutions (menthol, strawberry, tobacco, and vanilla) and unflavored solvents: propylene glycol (PG) or vegetable glycerin (VG). Viability was assessed by lactate dehydrogenase assay. Brochodilation and vasoreactivity were determined on isolated ovine bronchial rings (BR) and pulmonary arteries (PA). Results: Neither PG or VG impacted viability of immature or adult cells however, exposure to menthol and strawberry flavored solutions increased cell death. Neonatal cells were uniquely susceptible to menthol flavoring-induced toxicity, and all four flavorings demonstrated lower lethal doses (LD50) in immature PASMC. Exposure to flavored solutions induced bronchodilation of neonatal BR, while only menthol induced airway relaxation in adults. In contrast, PG/VG and flavored solutions did not impact vasoreactivity with the exception of menthol-induced relaxation of adult PAs. Conclusion: The immature lung is uniquely susceptible to cellular toxicity and altered airway responses with exposure to common flavored e-cigarette solutions. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1660-4601 1661-7827 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07bb69c7e7531dac6fde3b4d7be3a865Test http://europepmc.org/articles/PMC6801380Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....07bb69c7e7531dac6fde3b4d7be3a865 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16604601 16617827 |
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