التفاصيل البيبلوغرافية
| العنوان: |
Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes. |
| المؤلفون: |
Hee-Young Park1 hypark@bu.edu, Wu, Christina1, Yaar, Mina1, Stachur, Christina M.1, Kosmadaki, Marita1, Gilchrest, Barbara A.1 |
| المصدر: |
International Journal of Cell Biology. 2009, Vol. 2009, p1-11. 11p. 3 Black and White Photographs, 4 Graphs. |
| مصطلحات موضوعية: |
*MELANOCYTES, *EPITHELIAL cells, *BONE morphogenetic proteins, *PHENOL oxidase, *PHOSPHORYLATION, *GROWTH factors, *TRANSCRIPTION factors, *PROTEIN research |
| مستخلص: |
Bone Morphogenetic Protein (BMP-4) was shown to down-regulate melanogenesis, in part, by decreasing the level of tyrosinase [Yaar et al. (2006) JBC:281]. Results presented here show that BMP-4 down-regulated the protein levels of TRP-1, PKC-β, and MCI-R. When paired cultures of human melanocytes were treated with vehicle or BMP-4 (25 ng/ml), MAPK/ERK were phosphorylated within one hour of BMP-4 treatment. Then the activated MAPK/ERK caused an acute phosphorylation of MITF, followed by proteosome-mediated degradation of MITF, the key transcription factor for melanogenic proteins [Wu et al. (2000) Gene & Development:14]. However, prolonged exposure of melanocytes to BMP-4 (up to 48 hours) caused a decrease in the level of MITF-M transcript. In addition, BMP-4 decreased the intracellular level of cAMP, the key regulator of MITF expression. These results demonstrate that BMP-4 activates MAPK/ERK signaling pathway to transiently activate MITF; however, chronic treatment of BMP-4 to melanocytes causes a down-regulation of the expression of MITF, possibly in a cAMP-dependent pathway. [ABSTRACT FROM AUTHOR] |
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