Direct cytolysis by partially-purified preparations of immune interferon
| العنوان: | Direct cytolysis by partially-purified preparations of immune interferon |
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| المؤلفون: | Stephen K. Tyring, W. Robert Fleischmann, Gary R. Klimpel, Samuel Baron |
| المصدر: | International Journal of Cancer. 30:59-64 |
| بيانات النشر: | Wiley, 1982. |
| سنة النشر: | 1982 |
| مصطلحات موضوعية: | Cancer Research, Hot Temperature, Lysis, Cell Survival, Cell Count, Viral Plaque Assay, Biology, Antibodies, Cell Line, Mice, Species Specificity, Interferon, medicine, Animals, Humans, Leukemia P388, Cell growth, Hydrogen-Ion Concentration, Molecular biology, Titer, Cytolysis, Cell killing, Oncology, Cell culture, biology.protein, Interferons, Antibody, medicine.drug |
| الوصف: | Mouse IFN gamma preparations purified 30-fold were found to have direct cytolytic activity against a number of tumor and normal cells. Cell killing was determined using a sensitive, rapid and accurate assay which employed very low numbers of cells and very small quantities of interferon. The cytolytic activity of IFN gamma on 11 murine tumor cell lines was investigated. A 20-fold difference was found between the most-sensitive cell type, P-388 lymphoma, versus the most resistant cell type, C127v leukemia. A number of normal mouse cells was also found to have low to intermediate sensitivity to the cytolytic action of IFN gamma. Human IFN gamma was also shown to have cytolytic activity which, like mouse IFN gamma, was relatively species-specific. Direct cytolysis was not found to be a characteristic of IFN-alpha/beta. Different mechanisms of action for the antiviral and cytolytic activities of IFN gamma are indicated because the cytolytic titer of IFN gamma did not parallel its antiviral titer on most cell types and increasing the cell number produced a decrease in the cytolytic titer and an increase in the anti-viral titer. High concentrations of IFN gamma (i.e., 2,900 units/ml) resulted in complete lysis of cells within 24 h, while lower concentrations (i.e., 700 units/ml) resulted in a reversible inhibition of cell growth during this time period. Evidence that the cytolytic substance in the IFN gamma preparation was IFN gamma include the following: (1) both antiviral and anticellular activities copurified through a 30-fold purification; and both activities were (2) relatively species-specific; (3) sensitive to heat; (4) inactivated by low pH and (5) neutralized by antibodies to IFN gamma. Therefore, we propose the possibility that direct cytolysis is yet another of IFN gamma's distinctive antivities. |
| تدمد: | 1097-0215 0020-7136 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78c89b73fa14587f7d30e0d93907fe1bTest https://doi.org/10.1002/ijc.2910300111Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....78c89b73fa14587f7d30e0d93907fe1b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
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