Granulocyte-macrophage colony-stimulating factor induces human melanoma-cell migration
| العنوان: | Granulocyte-macrophage colony-stimulating factor induces human melanoma-cell migration |
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| المؤلفون: | Elise C. Kohn, Gambrill H. Hollister, Lance A. Liotta, Elliott Schiffmann, Sharon Wahl, John D. Dipersio |
| المصدر: | International Journal of Cancer. 53:968-972 |
| بيانات النشر: | Wiley, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Cancer Research, Autocrine Motility Factor, medicine.medical_treatment, Biology, Pertussis toxin, Sensitivity and Specificity, Cell Movement, Tumor Cells, Cultured, medicine, Humans, Melanoma, Chemotactic Factors, Granulocyte-Macrophage Colony-Stimulating Factor, Cell migration, Chemotaxis, medicine.disease, Recombinant Proteins, Granulocyte macrophage colony-stimulating factor, Cytokine, Oncology, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor, Cell culture, Immunology, Cancer research, Signal Transduction, medicine.drug |
| الوصف: | Tumor metastasis is the primary cause of death for cancer patients. The metastatic cascade requires successful tumor cell invasion into and through vascular and parenchymal barriers. We have shown that autocrine motility factor (AMF, autotaxin) and the insulin-like growth factors (IGFs) induce tumor-cell migration. Since granulocyte-macrophage colony-stimulating factor (GM-CSF) has been shown to prime neutrophils for chemotaxis, we have therefore studied the influence of GM-CSF upon tumor cells and report that GM-CSF stimulates migration of these cells in a dose-dependent fashion. The ED50 for A2058 human melanoma cell line chemotaxis to GM-CSF is approx. 60 pM. The motile response to GM-CSF was additive to that of IGF-I and AMF, both of which are potent attractants for tumor cells. Pre-treatment of cells for 2 hr with non-toxic concentrations of pertussis toxin (PT) or amiloride resulted in a 50% inhibition of chemotaxis to GM-CSF. Therefore, GM-CSF, through PT- and amiloride-sensitive signal pathways, is a potent attractant for melanoma cells, the response to which is additive to that of other attractants. The presence of the GM-CSF receptor in A2058 melanoma cells was indicated by Northern-blot analysis which identified message transcripts of 2.1 and 3.0 kb. These data emphasize the versatility of the melanoma cell migration response to an array of cytokines, including GM-CSF. |
| تدمد: | 1097-0215 0020-7136 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10476c3b920ee7f2178cde48c23c8143Test https://doi.org/10.1002/ijc.2910530618Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....10476c3b920ee7f2178cde48c23c8143 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
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