المؤلفون: Anna S. Kadish, Donald M. Marcus, Barry R. Bloom

المصدر: International Journal of Cancer. 18:581-586

مصطلحات موضوعية: Adult, Cancer Research, medicine.medical_specialty, Leukocyte migration, Breast Neoplasms, Cross Reactions, Breast Diseases, Epitopes, Breast cancer, Antigen, Antigens, Neoplasm, Leukocytes, medicine, Humans, Aged, business.industry, Cancer, Middle Aged, medicine.disease, Fibrocystic disease, Molecular biology, Surgery, Oncology, Cell Migration Inhibition, Chromatography, Gel, Female, Breast disease, Adenofibroma, business, Human breast, Autologous tumor

الوصف: The existence of CMI to tumor-associated antigens present in 3 M KCl extracts of breast carcinomas was demonstrated in a group of breast cancer patients by the leukocyte migration inhibition (LMI) assay. When crude KCl extracts were tested, 3 of 5 breast cancer patients gave a positive response to autologous tumor extracts. Eleven of 20 gave a positive response to allogeneic extracts as compared to 3 of 22 controls (including 6 patients with benign breast disease, 7 with non-mammary cancers and 9 normal controls). Extracts of fibrocystic disease tissue gave positive LMI tests in 2 of 5 breast cancer patients, suggesting the presence of antigenic cross-reactivity between benign and malignant breast disease. An extract of a medullary carcinoma of breast was fractionated on Sephadex G-200 and the effluent pooled into three fractions. The high molecular weight fraction produced LMI in 11 of 22 breast cancer patients and in 1 of 19 controls, including patients with benign breast disease, other cancers and normal individuals. The low molecular weight fraction produced LMI in both the benign (4 of 6) and the malignant breast disease (6 of 20) patients, but not in the controls (0 of 12). A simple fractionation technique has thus separated “cancer-specific” from “organspecific” activity. Sephadex G-200 fractions were active at a much lower protein concentration than the crude 3 M KCl extracts. Inhibition De La Migration Des Leucocytes Par Des Antigenes Associes au Cancer Mammaire Humain Une immunite a mediation cellulaire (CMI) contre les antigenes associes a la tumeur que contiennent des extraits (3 M KCl) de cancers mammaires a ete mise en evidence chez des malades atteintes de ce cancer au moyen de l'epreuve d'inhibition de la migration des leucocytes (LMI). Lorsque l'on a teste les extraits bruts, 3 malades sur 5 ont reagi positivement aux extraits de tumeur autologue. Onze malades sur 20 ont reagi positivement aux extraits allogeniques, de měme que 3 temoins sur 22 (6 cas d'affections mammaires benignes, 7 cas de cancers non mammaires et 9 temoins normaux). Avec les extraits de tissus de personnes souffrant de maladie fibrokystique, les tests LMI ont ete positifs dans 2 cas de cancer du sein sur 5, ce qui donne a penser qu'il existe une reactivite antigenique croisee entre les affections mammaires benignes et malignes. Un extrait d'epithelioma encephaloide du sein a ete fractionne sur Sephadex G-200; l'effluent a donne trois fractions. La fraction de poids moleculaire eleve a produit une LMI chez 11 des 22 malades atteintes de cancer du sein et chez 1 des 19 temoins (affections mammaires benignes, autres cancers et personnes normales). La fraction de faible poids moleculaire a induit une LMI dans les cas d'affection mammaire benigne (4 sur 6) et maligne (6 sur 20), mais pas chez les temoins (0 sur 12). Une simple technique de fractionnement a donc separe l'activite “cancerospecifique” de l'activite “organospecifique”. Par comparaison avec les extraits bruts au KCl, les fractions obtenues sur Sephadex G-200 etaient actives a une concentration proteique beaucoup plus faible.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96a006ffd503f193802443c7ac469967Test
https://doi.org/10.1002/ijc.2910180506Test

المؤلفون: Branko Malenica, Renata Mažuran, Hamza Mujagić, Vlatko Silobrčić

المصدر: International Journal of Cancer. 17:14-20

مصطلحات موضوعية: Adult, Male, Cancer Research, Cellular immunity, Adolescent, Breast Neoplasms, melanoma, cellular immunity, monocyte spreading inhibition, Biology, Peripheral blood mononuclear cell, Monocytes, Antigen, Antigens, Neoplasm, medicine, Humans, Melanoma, neoplasms, Aged, Immunity, Cellular, Melanoma-associated antigen, Monocyte, Carcinoma, Sarcoma, Middle Aged, medicine.disease, In vitro, medicine.anatomical_structure, Oncology, Cell Migration Inhibition, Immunology, Female, Breast carcinoma

الوصف: In vitro inhibition of monocyte spreading (a correlate of cellular immunity) was used to detect cell-mediated immune reactions of melanoma patients to specific melanoma antigens. Two soluble preparations of human melanoma antigens (MA-1 and MA-2) and one of a breast carcinoma (BCA) were prepared. The preparations were incubated in vitro with mononuclear cells isolated from the blood of 24 patients with melanoma, six patients with malignancies other than melanoma and 14 healthy donors. Spreading of monocytes from healthy donors was not inhibited by either MA-1 and MA-2 or BCA. MA-1 and MA-2 significantly inhibited the spreading of monocytes from patients with melanoma, while monocytes from patients with other malignancies were not affected. Spreading of monocytes from patients with melanoma was inhibited by the preparation of BCA. We conclude that inhibition of monocyte spreading can detect, in vitro, a cellular immune reaction to specific melanoma antigens in patients with melanoma.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe0b1a1698e30dd1eb6a745a9ffbfdb1Test
https://doi.org/10.1002/ijc.2910170104Test

المؤلفون: O. Eremin, J. Ashby, J. P. Stephens

المصدر: International Journal of Cancer. 21:35-41

مصطلحات موضوعية: Adult, Cytotoxicity, Immunologic, Cancer Research, Pathology, medicine.medical_specialty, Lymphocyte, Palatine Tonsil, Cell, Blood Donors, Breast Neoplasms, Biology, Immunoenzyme Techniques, medicine, Humans, Lymph node, Aged, Middle Aged, Hyperplasia, Cytotoxicity Tests, Immunologic, medicine.disease, Killer Cells, Natural, Axilla, medicine.anatomical_structure, Lymphatic system, Oncology, Lymphatic Metastasis, Tonsil, Cell Migration Inhibition, Female, Lymph Nodes, Lymph

الوصف: Natural cytotoxicity against CLA-4 and D6 target cells was determined in the peripheral blood of healthy donors and women with mammary carcinoma (localized to the breast and axilla), and in human normal and regional tumour lymph nodes. The NK cell activity in the blood of women with clinically localized mammary carcinoma showed a wide range of reactivities but was similar to the pattern detected in healthy controls. The NK cell activity in human normal lymph nodes also showed a wide range and was the same irrespective of the anatomical site of origin of the lymph node. Lymph nodes draining a variety of solid tumours had a similar spectrum of NK cell activity, there being no obvious correlation between the level of NK cell activity and such parameters as size and histological type of tumour, hyperplasia or metastatic spread in the regional node. Trypsin treatment of lymphocyte suspensions from the various lymphoid compartments suggested that the cell responsible for natural cytotoxicity in blood was probably different from the NK cell in tonsil and lymph nodes.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8cd6308373c167f4086e42cab66b1614Test
https://doi.org/10.1002/ijc.2910210108Test

المؤلفون: Margaret D. Connell, Catherine E. Ross, Deirdre E. Hoyle, Rona M. MacKie, A. J. Cochran, Alan M. Jackson, Robert M. Grant, Gavin Sandilands, Keith Whaley

المصدر: International Journal of Cancer. 18:298-309

مصطلحات موضوعية: Adult, Male, Cancer Research, Antigens, Fungal, Adolescent, Fluorescent Antibody Technique, Immunoglobulins, Breast Neoplasms, Lymphocyte Activation, Complement components, Lymphocyte subpopulations, Antigen, Antigens, Neoplasm, Leukocytes, medicine, Humans, Lymphocytes, Melanoma, Aged, Skin Tests, business.industry, Cell Membrane, Immune adherence, Cancer, Complement System Proteins, Middle Aged, Cytotoxicity Tests, Immunologic, medicine.disease, Immune Adherence Reaction, Oncology, Cell Migration Inhibition, Immunology, Serum inhibition, Female, Mitogens, Immunocompetence, business, Leucocyte migration

الوصف: We have examined 111 cancer patients and 111 control individuals for general immunocompetence (haematological values, “recall” antigen skin tests, PHA and PPD induced lymphocyte transformation, serum Ig levels and lymphocyte subpopulations), for evidence of sensitisation to tumour-associated anti-tigens (leucocyte migration test, serum inhibition of autologous leucocyte migration, lymphocytotoxicity, membrane immunofluorescence and immune adherence) and for evidence of continuing immune reactions (alterations of complement components and anti-complementary activity). Major differences between the cancer patients and controls were demonstrated by several tests of sensitisation and these also detected differences between patients with and without metastases. The only differences detected between cancer patients and controls by the tests of general immunocompetence were in serum IgG and IgA (higher in the cancer patients) and lymphocyte subpopulations (“active” T, autorosetting lymphocytes and lymphocytes forming “super-rosettes” increased in cancer patients). In a comparison of cancer patients with and without metastases, patients with metastases were less often reactive to the Candida DHS and streptokinase-streptodornase antigens and had raised circulating Fc positive cells. Abnormalities of the individual components of complement occurred in about half the cancer patients, but were equally common in those with and without metastases. Serum anti-complementary activity was very rarely detected. The tests of specific sensitisation correlated reasonably well but correlations of tests of general immunocompetence were infrequent. Une etude de l'immunologie des malades cancereux Nous avons examine 111 cancereux et 111 temoins du point de vue de leur immunocompetence generale (valeurs hematologiques, tests cutanes avec des antigenes “de rappel”, transformation des lymphocytes induite par les PHA et PPD, niveau d'lg dans le serem et sous-populations de lymphocytes), de leur sensibilisation aux antigenes associes a la tumeur (epreuve de migration des leucocytes, inhibition seique de la migration des leucocytes autologues, lymphocytotoxicitd, immunojuorescence de la membrane et immuno-adhdrence), et de la persistance de leurs reactions immunitaires (alterations des composants du complement et activite anticomplimentaire). Plusieurs tests de sensibilisation ont mis en tvidence des diffirences importantes entre les cancereux et les temoins ainsi quentre les malades presentant des metastases ou n'en presentant pas. Les seules differences decelees entre 1es cancereux et les temoins au moyen des tests d'immunocompttence gPnPrale concernaient 1'IgG et 1'IgA seriques (plus elevees chez les canceeux) ainsi que les sous-populations de lymphocytes (les celfules T “actives”, les lymphocytes formant des autorosettes et les lymphocytes formant des “super-rosettes” sont plus nombreux chez les cancereux). Lors d'une comparaison entre les cancereux avec ou sans metastases, on a constate que les premiers reagissent moins frequemment aux antigenes Candida DHS et Streptokinase-streptodornase et qu'ils possedent davantage de cellules circulantes Fc-positives. Des anomalies des composants du complement ont ete observees chez la moitie environ des cancereux, mais elles etaient aussi frequentes chez ceux qui presentaient des metastases que chez ceux qui n'en presentaient pas. On a tres rarement decele une activite anti-complementaire dans les serums. Les tests de sensibilisation specifique sont en assez bonne correlation mais on a rarement observe des correlations entre les tests d'immunocompetence generale.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34c3f64e8b645241ab1522d47783889eTest
https://doi.org/10.1002/ijc.2910180306Test

المؤلفون: Jytte Roed-Petersen, Bjarne Roed-Petersen, Gunnar Bendixen

المصدر: International Journal of Cancer. 12:66-72

مصطلحات موضوعية: Adult, Male, Cancer Research, Leukocyte migration, Pathology, medicine.medical_specialty, Normal oral mucosa, Biopsy, Biology, Antigen, Antigens, Neoplasm, Leukocytes, medicine, Humans, Hypersensitivity, Delayed, Aged, Immunity, Cellular, Periodic Acid, Mouth Mucosa, Middle Aged, Cell mediated immunity, Oral leukoplakia, stomatognathic diseases, Oncology, Cell Migration Inhibition, Immunology, Tissue extracts, Toxicity, Female, Leukoplakia, Oral

الوصف: Tissue extracts from 11 oral leukoplakias and from normal oral mucosa of the same patients were tested in the LMT against autologous leukocytes. Five patients showed inhibition of the migration with leukoplakial tissue and one showed inhibition with normal mucosa. The migration of leukocytes from matched controls was not influenced by the tissue extracts, which accordingly had no non-specific toxicity. It is concluded that oral leukoplakia is associated with a state of tissue-specific, cellular hypersensitivity against antigenic components of the leukoplakial tissue.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c989d5c2677597b3665d23f3bb8bd2eTest
https://doi.org/10.1002/ijc.2910120107Test

المؤلفون: A Arndt, K Rieche, G Pasternak

المصدر: International journal of cancer. 17(2)

مصطلحات موضوعية: Adult, Cancer Research, Leukocyte migration, Cellular immunity, Pathology, medicine.medical_specialty, Breast Neoplasms, Cross Reactions, Antigen, Antigens, Neoplasm, medicine, Leukocytes, Neoplasm, Humans, Aged, Immunity, Cellular, business.industry, Cancer, Cell Migration Inhibition, Middle Aged, medicine.disease, Tumor antigen, Oncology, Immunology, Female, business, Progressive disease, Follow-Up Studies

الوصف: In 58 cases of mammary cancer treated by surgery the leukocyte migration test (LMT) has been applied to the study of cellular immunity using homogenate from autochthonous and homologous tumors as antigens. A positive test, i.e. inhibition of migration by antigen, was observed in 52 patients from 1 day up to 40 days after surgery. Six patients were negative. There was extensive immunological cross-reactivity among mammary cancer in that a positive test was also obtained with homogenate from homologous mammary tumors. Generally, antigens from other tumors did not react. The test was also negative when the leukocytes were derived from healthy persons. Sera from mammary cancer patients abrogated the inhibitory effect of tumor antigen. The disappearance of positivity about 40 days after surgery and its reappearance during the metastatic process indicates that the positive LMT is associated with progressive disease.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121ab71969dd8d5c1c0f42370889d33bTest
https://pubmed.ncbi.nlm.nih.gov/1248905Test

المؤلفون: Lindsay Morrison, Claire, Catherine E. Ross, Rona M. MacKie, A. J. Cochran, G. Garland, Gaye Todd

المصدر: International journal of cancer. 24(1)

مصطلحات موضوعية: Adult, Cancer Research, Leukocyte migration, Pathology, medicine.medical_specialty, Cytological Techniques, Stage ii, Metastatic tumours, Antigen, Formaldehyde, medicine, Leukocytes, Humans, In patient, Stage (cooking), Neoplasm Metastasis, Melanoma, Cells, Cultured, Melanoma patient, business.industry, medicine.disease, Culture Media, Oncology, Lymphatic Metastasis, Cell Migration Inhibition, business

الوصف: Leukocytes from 72 melanoma patients and 75 control donors were examined in a two-stage (indirect) leukocyte migration assay, using formalin-fixed melanoma cells (FMC) and control, normal or non-melanoma tumour cells (FCC) as the source of antigen. Inhibitory supernatants were produced significantly more often by cultures of melanoma leukocytes and FMC than by melanoma leukocytes and FCC or control leukocytes and FMC or FCC. Reactive leukocytes were most frequently derived from stage II patients (65%), followed by stage I patients with tumour present (58%), stage III patients (29%) and stage I patients without detectable tumour (20%). The likelihood that a melanoma patient's leukocytes would react increased progressively with the number of different FMC preparations tested. A similar, though less steep increase in reactivity was seen with control donor leukocytes and different FMC preparations. No significant increase in reaction frequency was seen with melanoma or control leukocytes exposed to increasing numbers of different FCC preparations. Significant discrimination between melanoma patients' and control donors' leukocytes was achieved with FMC from both primary and metastatic tumours. Concordance of positivity or negativity was seen in 60% of concurrent one-stage and two-stage leukocyte migration assays.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f5ecf2bc5bf788ef2e9cefcdfd34fd2Test
https://pubmed.ncbi.nlm.nih.gov/383631Test

المؤلفون: Morrison Lj, Chan Cw, Rona M. Mackie, Alistair J. Cochran, Kennedy R, G. Todd

المصدر: International journal of cancer. 26(3)

مصطلحات موضوعية: Adult, Male, Cancer Research, Leukocyte migration, Adolescent, Bacillus, In Vitro Techniques, Neoplasms, medicine, Leukocytes, Humans, Hypersensitivity, Delayed, Aged, biology, business.industry, Cancer stage, Age Factors, Cancer, Middle Aged, biology.organism_classification, medicine.disease, Mycobacterium bovis, In vitro, Oncology, Immunology, Cell Migration Inhibition, Female, business

الوصف: We examined the in vitro reaction of leukocytes from 71 cancer patients and 121 control donors (disease-free individuals or patients with non-malignant non-debilitating conditions) against Bacillus Calmette-Guerin using a one-stage capillary leukocyte migration assay. The proportion of reactive cancer patients and control donors increased with increasing BCG concentration, but the proportion of patients who reacted was less than that of control donors at all concentrations of BCG. As cancer stage advanced and the volume of clinically detectable tumor increased, the proportion of reactive patients decreased. There was a small age-related decline in reactivity in the control population. The results of simultaneous in vitro tests and Mantoux reactions were concurrent in a majority of normal individuals so tested.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07656374e89582de06d5de630168da0eTest
https://pubmed.ncbi.nlm.nih.gov/7026459Test

المؤلفون: Michael P. Alpers, Peter J. Fimmel, Ewart M. Mackay-Scollay, Patrick K. Lai, David Keast

المصدر: International journal of cancer. 13(5)

مصطلحات موضوعية: Adult, Male, Cancer Research, Leukocyte migration, Cellular immunity, Herpesvirus 4, Human, Adolescent, Lymphoid Tissue, Fluorescent Antibody Technique, medicine.disease_cause, Antibodies, Viral, Virus, Epithelium, Cell Line, Antigen, hemic and lymphatic diseases, medicine, Leukocytes, Animals, Humans, Amnion, Antigens, Viral, Lung, Infectivity, Immunity, Cellular, biology, Infant, Newborn, Epithelial Cells, Fibroblasts, Epstein–Barr virus, Virology, Raji cell, Oncology, Immunology, Cell Migration Inhibition, biology.protein, Cattle, Female, Antibody

الوصف: Leukocyte migration inhibition was employed to study cell-mediated immunity (CMI) to Epstein-Barr virus (EBV) in 29 normal subjects. Anti-EBV capsid antibody titres in these subjects ranged from 0 to 1:160. Extracts of seven human lymphoblastoid cell lines carrying EBV were evaluated as the source of test antigen. Of these cell lines studied, only P3HR-1 proved suitable as a source of EBV antigen for use in the test. Undiluted tissue-culture supernatant fluids of the EBV-carrying cell lines P3HR-1 and SH-T1 caused only weak or equivocal migration inhibition in normal subjects; others tested were without effect. Antigen controls consisted of extracts of the Raji cell line, which contains the EBV genome but produces no EBV antigen, and cultured human cells with no known association with EBV, all of which failed to induce migration inhibition. Cellular controls consisted of EBV sero-negative adult leukocytes and foetal cord blood leukocytes which did not respond to P3HR-1 extract, although two of the five cord sera contained anti-EBV capsid antibody. The migration inhibition response was antigen-dose-dependent and was not affected by anti-EBV antibody capable of neutralizing the infectivity of EBV. These results show that CMI to EBV is present in normal individuals who have antibody to EBV capsid antigen.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc1b277637b6cfcaf20fbe7821d89837Test
https://pubmed.ncbi.nlm.nih.gov/4367339Test

المؤلفون: J Genin, Lacour F, Aneta Segall, Odette Weiler, Jean Lacour

المصدر: International journal of cancer. 9(2)

مصطلحات موضوعية: Adult, Male, Cancer Research, Pathology, medicine.medical_specialty, Cellular immunity, Leukocyte migration, Skin Neoplasms, Adolescent, Fibrosarcoma, Adenoma, Pleomorphic, Bone Neoplasms, Breast Neoplasms, Dysgerminoma, In Vitro Techniques, Peripheral blood mononuclear cell, Antigen, Immunity, Neoplasms, Rhabdomyosarcoma, Carcinoma, Leukocytes, Medicine, Humans, Child, Aged, Ovarian Neoplasms, Immunity, Cellular, Osteosarcoma, business.industry, Tissue Extracts, Lymphoma, Non-Hodgkin, Mesenchymal stem cell, Liposarcoma, Middle Aged, medicine.disease, Hodgkin Disease, Tumor antigen, Oncology, Cell Migration Inhibition, Carcinoma, Squamous Cell, Female, Lymphoma, Large B-Cell, Diffuse, business, Neurilemmoma

الوصف: A study of cell-mediated immunity has been undertaken in vitro by the leukocyte migration test (LMT) on 57 patients with operable carcinoma. Tumor extracts induced an inhibition of autologous leukocyte migration in 58% of the cases: 10 out of 17 lymphomas, 10 out of 15 malignant mesenchymal tumors, 8 out of 13 mammary carcinomas, 2 out of 4 melanomas, and 3 out of 8 various other tumors. Migration of mononuclear cells was especially inhibited but not that of neutrophils. Thus a previous sensitization of the lymphocytes by tumor antigen (s) has been shown. On the contrary, 9 extracts of benign and 17 extracts of normal tissue did not significantly inhibit the migration of autologous cells. Almost all tumor extracts were tested against allogeneic leukocytes from patients bearing the same or a histologically different type of tumor, or from healthy subjects (101 cases). Cross-reactions were observed in five melanomas, in one case of Hodgkin's disease, and in one breast carcinoma. In all but two cases, inhibition of leukocyte migration did not occur in patients with a different type of tumor or in non-cancerous patients. It is too early to judge the possible prognostic value of a positive LMT showing a state of cellular hypersensitivity against tumor antigens. Only a survival time analysis and a wider follow-up study of the “immunological balance” during the course of the disease can provide an answer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba3855700d441dca422e883196115b84Test
https://pubmed.ncbi.nlm.nih.gov/4339416Test