دورية أكاديمية

Evaluation of cytotoxicity, reactive oxygen species and nitrous oxide of nanochitosan from shrimp shell.

التفاصيل البيبلوغرافية
العنوان: Evaluation of cytotoxicity, reactive oxygen species and nitrous oxide of nanochitosan from shrimp shell.
المؤلفون: Pompeu, Lenise Deon1 (AUTHOR), Viana, Altevir Rossato1 (AUTHOR), Fernandes, Liana da Silva1 (AUTHOR), da Silva, William Leonardo1 (AUTHOR) w.silva@ufn.edu.br
المصدر: International Journal of Biological Macromolecules. Apr2023, Vol. 235, pN.PAG-N.PAG. 1p.
مصطلحات موضوعية: *CHITIN, *REACTIVE oxygen species, *CHITOSAN, *POINTS of zero charge, *NITROUS oxide, *ENERGY dispersive X-ray spectroscopy, *FOURIER transform infrared spectroscopy
مستخلص: This work aims to synthesize, characterize and evaluate the biological activity of nanochitosan (NQ) prepared from shrimp, showing an innovative character and correlating with sustainable development, in promoting an alternative to the solid waste (shrimp) shell and a biological application of the novel nanomaterial. The NQ synthesis was carried out by the alkaline deacetylation process of chitin obtained of the demineralization, deproteinization and deodorization steps from shrimp shells. NQ was characterized by X-ray Powder Diffraction (XRD), Fourier Transform infrared spectroscopy (FTIR), Scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDS), N 2 porosimetry (BET/BJH methods), zeta potential (ZP) and zero charge point (pH ZCP). To evaluate the safety profile was carried out the cytotoxicity, DCFHA and NO tests in 293T and HaCat cell lines. Regarding the cell viability, NQ did not show toxicity for the tested cell lines. In the evaluation of the ROS production and NO tests, there was no increase in the levels of free radicals and between the negative control, respectively. Therefore, NQ does not present cytotoxicity in the cell lines tested (10, 30, 100 and 300 μg mL−1), proposing new perspectives on the use of NQ as a potential nanomaterial for biomedical applications. • Nanochitosan was synthesized from shrimp shells by the synthesis process. • The cytotoxicity of nanochitosan in cell culture was evaluated through the cell line 293T and HaCat. • To evaluate the production of reactive oxygen species (ROS) and nitric oxide (NO) [ABSTRACT FROM AUTHOR]
قاعدة البيانات: Academic Search Index
الوصف
تدمد:01418130
DOI:10.1016/j.ijbiomac.2023.123730