التفاصيل البيبلوغرافية
| العنوان: |
Effects of sulfated polysaccharide from Masson pine (Pinus massoniana) pollen on the proliferation and cell cycle of HepG2 cells |
| المؤلفون: |
Chu, Hui-Li1,2, Mao, Hua2, Feng, Wei2, Liu, Jing-wen3, Geng, Yue2 yuegeng@sohu.com |
| المصدر: |
International Journal of Biological Macromolecules. Apr2013, Vol. 55, p104-108. 5p. |
| مصطلحات موضوعية: |
*POLYSACCHARIDES, *POLLEN extract, *CELL cycle, *CELL proliferation, *LIVER cancer, *LIVER cells, *TETRAZOLIUM compounds, *PINE |
| مستخلص: |
Abstract: Aim: To explore the inhibitory effect of sulfated polysaccharide from Masson pine (Pinus massoniana) pollen (SPPM60) on G2/M phase of human liver cancer HepG2 cells and its mechanism. Methods: The proliferation rate of HepG2 cells was evaluated by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The cycles of HepG2 cells were measured by flow cytometry when 200μg/ml concentration of SPPM60 was adopted, the expression of the genes related to cell cycle was detected by real-time PCR. Results: SPPM60 inhibited the proliferation of HepG2 cells and the inhibition rate was elevated with increase of SPPM60 concentration. After treatment with 200μg/ml of SPPM60, the percentage of S phase cells was decreased, but that of G2/M phase was significantly increased (72h vs control: 32.96±0.33% vs 18.59±0.04%, 3.44±0.05% vs 18.30±0.08%, P <0.01). The results of real-time PCR showed that SPPM60 could down-regulate the mRNA levels of CDK1 and CyclinB (P <0.01), and up-regulate the expression of p53 and p21 (P <0.05). Conclusion: SPPM60 causes arrest of HepG2 cells at G2/M phase, and the mechanism is related to the down-regulation of CDK1 and CyclinB and up-regulation of p53 and p21 expression. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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