التفاصيل البيبلوغرافية
| العنوان: |
Time-dependent changes in the glycolytic pathway in activated T cells are independent of tumor burden or anti-cancer chemotherapy. |
| المؤلفون: |
Khalil, Sohaila M.1,2 (AUTHOR) Sohaila_galal@science.tanta.edu.eg, Eltaramsy, Asmaa3 (AUTHOR), Hegazi, Mona M.3 (AUTHOR), Mohamed, Tarek M.4 (AUTHOR), Alwasel, Saleh5 (AUTHOR), Salem, Mohamed L.1,2 (AUTHOR) mohamed.labib@science.tanta.edu.eg |
| المصدر: |
International Immunopharmacology. Sep2023, Vol. 122, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*EHRLICH ascites carcinoma, *T cells, *GLYCOLYSIS, *CANCER chemotherapy, *BIOTRANSFORMATION (Metabolism), *CELL physiology, *LACTATE dehydrogenase |
| مستخلص: |
• In resting lymphocyte, the activity of cells is low, therefore, the level of glucose is decreased, concomitant with a high level of G6P, HKase, PFKase and low levels of LDH and G6PD. • The activation of T-lymphocytes whether by antigen (tumor/chemotherapy) or by Con– A/IL2. • Short activation naive and early stage of tumor with or without chemotherapy can give high glycolic rate. However, late stage of tumor without chemotherapy gives low glycolic rate. • In late activation naive and early stage of tumor with chemotherapy can give high glycolic metabolism. These results are one of great importance application of adaptive T cell therapy. Although activated adoptive T cells therapy (ATC) is an effective approach for cancer treatment, it is not clear how modulation of T cell activation impacts their biochemical signature which significantly impacts the cell function. This study is aimed to investigate the impact of polyclonal activation on the metabolic signature of T cells from tumor-bearing mice under different settings of treatment with chemotherapy. Thirty female Swiss albino mice were divided into 5 groups (n = 6/each), Gp1(PBS), groups Gp2 were inoculated intraperitoneal (i.p) with 1 × 106 cells/mouse Ehrlich ascites carcinoma (EAC), Gp3-Gp5 were treated with cisplatin (20 mg/mice) which were represented as EAC/CIS/1wk Or EAC/CIS/2wk 3 times every other day. Splenocytes were cultured in or presence of concanavalin-A (Con-A) and IL-2 for 24 h or 72 h, then cells were harvested, and processed to determine the enzyme activities of hexokinase (HK), phosphofructokinase (PFK), lactate dehydrogenase (LDH) and glucose 6 phosphate dehydrogenase(G6PD) enzymes. The results showed that before culture, T cells harvested from EAC/PBS/1wk of mice or inoculated with EAC/CIS/1wk showed higher activity in HK, PFK, LDH, and G6PH as compared to naive T cells. After 24, and 72 h of culture and activation, the enzyme activities in T cells harvested from EAC/CIS/2wk mice or EAC/CIS/3wk mice decreased compared with their control. The late stage of the tumor without chemotherapy gives a low glycolic rate. In late activation, naive and early stages of the tumor with chemotherapy can give high glycolic metabolism. These results show great significance as an application of adoptive T-cell therapy. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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