التفاصيل البيبلوغرافية
| العنوان: |
A functional soluble form of CTLA-4 is present in the serum of celiac patients and correlates with mucosal injury. |
| المؤلفون: |
Simone, Rita, Brizzolara, Renata, Chiappori, Alessandra, Milintenda-Floriani, Francesca, Natale, Clelia, Greco, Luigi, Schiavo, Mara, Bagnasco, Marcello, Pesce, Giampaola, Saverino, Daniele |
| المصدر: |
International Immunology; Sep2009, Vol. 21 Issue 9, p1037-1045, 9p, 1 Chart, 5 Graphs |
| مصطلحات موضوعية: |
CELIAC disease, GENETIC disorders, AUTOIMMUNE diseases, IMMUNOLOGY, DISEASE susceptibility |
| مستخلص: |
Celiac disease (CD) is a multifactorial disorder influenced by environmental, genetic and immunological factors. Increasing evidence showed CTLA-4 gene as an important susceptibility locus for autoimmune disorders. A native soluble cytotoxic T-lymphocyte-associated protein-4 (sCTLA-4), lacking of transmembrane sequence, has been described in several autoimmune diseases. We aimed to evaluate the presence of increased sCTLA-4 concentration in the serum of patients with CD and the possible immunoregulatory function. Blood samples were collected from 160 CD patients; sCTLA-4 levels were evaluated by ELISA, western blot and reverse transcription–PCR. The capability of serum sCTLA-4 to modulate T-lymphocyte proliferation in vitro was evaluated by two-way mixed leukocyte reaction assay. We demonstrated high levels of sCTLA-4 in serum of untreated celiac patients. Additionally, we observed that sCTLA-4 concentrations are related to gluten intake and that a correlation between autoantibodies to tissue transglutaminase and sCTLA-4 concentration exists. Moreover, sCTLA-4 levels correlate with the degree of mucosal damage. Conversely, no correlation between sCTLA4 levels and the HLA-related risk was observed. Finally, we show that sCTLA-4 from sera of CD patients displays functional activities. These results strongly suggest a regulation of sCTLA-4 synthesis depending on the presence or absence of dietary gluten and imply a possible immunomodulatory effect on cytotoxic T lymphocyte functions. In gluten-exposed patients, serum sCTLA-4 levels might provide insight about mucosal injury. [ABSTRACT FROM PUBLISHER] |
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| قاعدة البيانات: |
Complementary Index |
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