Biocompatibility of various root canal filling materialsex vivo
| العنوان: | Biocompatibility of various root canal filling materialsex vivo |
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| المؤلفون: | Paolo Baldissara, Roberto Scotti, M. A. Croce, R. Tiozzo, Candida Parisi |
| المساهمون: | R. SCOTTI, R. TIOZZO, C. PARISI, M.A. CROCE, P. BALDISSARA |
| المصدر: | International Endodontic Journal. 41:651-657 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Materials science, food.ingredient, Cytotoxicity test, Biocompatibility, Cell Survival, Gingiva, cytotoxicity test, endodontic filler, fibroblasts cultures, resin sealers, Composite Resins, BIOCOMPATIBILITY, Root Canal Filling Materials, CITOTOXICITY ASSAY, food, Humans, Viability assay, Composite material, General Dentistry, Incubation, Cells, Cultured, Chromatography, Anova test, fibroblasts culture, Fibroblasts, Gutta-Percha, Filler (animal food), Ex vivo |
| الوصف: | Aim To evaluate the biocompatibility of a resin-based endodontic filler (RealSeal) using the indirect cytotoxicity test. Methodology Human gingival fibroblasts were cultured ex vivo. Pellets of the materials to be tested were incubated for 24, 48, and 72 h at 37 °C under sterile conditions to obtain their eluates. The fibroblasts were exposed to either diluted (50%) or undiluted eluates for 24 h. A culture medium with foetal calf serum was added to the control wells. Cell viability was estimated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide method. The data concerning cell viability were statistically analyzed using one-way anova test and Bonferroni multiple comparisons test. Results Eluates obtained after 24 h of incubation with the resin filler did not reduce cellular viability. An increase in cellular viability, as compared with control cells, was observed in the gutta-percha group. The undiluted eluate from the polyether material was cytotoxic, causing an 82 ± 4% decrease in cellular viability. Eluates obtained after 48 h of incubation with the resin filler increased cellular viability, whereas the polyether significantly reduced viability. Gutta-percha did not cause any detectable change. After 72 h of incubation the eluate of the resin filler caused an increase in cellular viability, as did gutta-percha, whereas polyether caused a significant decrease. Conclusions RealSeal resin filler was nontoxic in this laboratory model. Further investigations are necessary to verify its usefulness in clinical applications. |
| وصف الملف: | STAMPA |
| تدمد: | 1365-2591 0143-2885 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1674dc3e656c7b0b17887c8a9fbd16d5Test https://doi.org/10.1111/j.1365-2591.2008.01403.xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1674dc3e656c7b0b17887c8a9fbd16d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652591 01432885 |
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