التفاصيل البيبلوغرافية
| العنوان: |
Production and characterization of casein hydrolysates with a high amino acid Fischer's ratio using immobilized proteases |
| المؤلفون: |
Pedroche, Justo1, Yust, María M.1, Lqari, Hassane1, Girón-Calle, Julio1, Vioque, Javier1, Alaiz, Manuel1, Millán, Francisco frmillan@cica.es |
| المصدر: |
International Dairy Journal. Jun2004, Vol. 14 Issue 6, p527. 7p. |
| مصطلحات موضوعية: |
*AMINO acids, *PROTEOLYTIC enzymes, *ENZYMES, *CARBOXYPEPTIDASES |
| مستخلص: |
A procedure using immobilized enzymes has been developed to obtain protein hydrolysates with a high Fischer''s ratio (branched-chain amino acids/aromatic amino acids (AAA)) from bovine casein. Pre-digestion with trypsin was followed by treatment with chymotrypsin, which generated a hydrolysate enriched in peptides with AAA at the carboxyl end. Carboxypeptidase A was then used to remove these AAA. A fraction, which represents 24% of total hydrolyzed proteins, with a Fischer''s ratio of 30.6, and Phe+Tyr content below 1.4% of the total, was purified from this hydrolysate by gel filtration in a Biogel P2 column. This material could be used for the treatment of patients suffering from certain hepatic encephalophaties, tyrosinemia and phenylketonuria. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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