An optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species
| العنوان: | An optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species |
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| المؤلفون: | Roldan Maria Ines, Vera Manuel, García-Marín José Luís, Heras Sandra, Planella Albi Laia |
| المساهمون: | Ministerio de Economía y Competitividad (Espanya) |
| المصدر: | © Integratize zoology, 2017, vol. 12, núm. 5, p. 421-427 Articles publicats (D-B) DUGiDocs – Universitat de Girona instname |
| بيانات النشر: | Wiley, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Genotype, ADN, Zoology, Ocean Engineering, Aquatic Science, Biology, Oceanography, Polymerase Chain Reaction, Antennatus, law.invention, 03 medical and health sciences, chemistry.chemical_compound, Penaeidae, law, Animals, Shrimps -- Genetics, Genotip, Genotyping, Polymerase chain reaction, Water Science and Technology, Genetics, Global and Planetary Change, Ecology, Reproduction, DNA, biology.organism_classification, Gambes -- Genètica, DNA extraction, Spermatogonia, Shrimp, genomic DNA, 030104 developmental biology, chemistry, Spermatophore, Female, Animal Science and Zoology, Génotype |
| الوصف: | The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality. Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species. However, all current protocols require invasive handling methods with males for DNA isolation. Using Aristeus antennatus as a model we tested a reliable non‐invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum. The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping. This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs. More importantly, it can be applied to identify the mating pairs and male structure in wild populations of species as A. antennatus, where males are often difficult to capture. Our method could be also valuable for biological studies on other spermatophore‐using species, such as myriapods, arachnids and insect This work was supported by a grant from Spanish Ministerio de Economía y Competitividad (CTM2014-54648-C2-2-R) to MIR. LP benefited from predoctoral fellowship from the Universitat de Girona (BR2014) |
| وصف الملف: | application/pdf |
| تدمد: | 1749-4877 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c29029d70978fdc29a4962c1d6c67e49Test https://doi.org/10.1111/1749-4877.12250Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c29029d70978fdc29a4962c1d6c67e49 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17494877 |
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