A proctolin-like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion
| العنوان: | A proctolin-like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion |
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| المؤلفون: | Vera I. D. Ros, Stefano Parenti, Yue Han, Angel Llopis-Giménez, Salvador Herrero |
| المصدر: | Insect Science, 29(1), 230-244 Insect Science 29 (2022) 1 |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | animal structures, media_common.quotation_subject, viruses, Laboratory of Virology, Neuropeptide, Insect, neuromodulator, neuropeptide, Spodoptera, Receptors for Activated C Kinase, Proctolin, General Biochemistry, Genetics and Molecular Biology, SeMNPV, Laboratorium voor Virologie, Exigua, Animals, Ecology, Evolution, Behavior and Systematics, media_common, biology, Effector, behavior, Neuropeptides, fungi, Wild type, biology.organism_classification, PE&RC, Cell biology, Autographa californica, beet armyworm, Larva, Insect Science, physiology, Digestion, Peptides, Baculoviridae, Oligopeptides, Agronomy and Crop Science, Locomotion |
| الوصف: | Baculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues, included the central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters, or neuromodulators. These peptides are involved in regulating animal physiology and behavior and could be altered after baculovirus infection. In this study, we have investigated the effect of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) infection on expression of Spodoptera exigua neuropeptides and neuropeptide-like genes. Expression of the gene encoding a polypeptide that resembles the well-known insect neuropeptide proctolin and named as proctolin-like peptide (PLP), was downregulated in the larval brain following infection and was chosen for further analysis. A recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) overexpressing the C-terminal part of the PLP was generated and used in bioassays using S. exigua larvae to study its influence on the viral infection and insect behavior. AcMNPV-PLP-infected larvae showed less locomotion activity and a reduction in growth compared to larvae infected with wild type AcMNPV or mock-infected larvae. These results are indicative of this new peptide as a neuromodulator that regulates visceral and skeletal muscle contractions and offers a novel effector involved in the behavioral changes during baculovirus infection. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 1672-9609 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0d6d6b0c738a954a537a82d4917957dTest https://doi.org/10.1111/1744-7917.12913Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....e0d6d6b0c738a954a537a82d4917957d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16729609 |
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