دورية
Campylobacter jejuniOuter Membrane Vesicles Play an Important Role in Bacterial Interactions with Human Intestinal Epithelial Cells
| العنوان: | Campylobacter jejuniOuter Membrane Vesicles Play an Important Role in Bacterial Interactions with Human Intestinal Epithelial Cells |
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| المؤلفون: | Elmi, Abdi, Watson, Eleanor, Sandu, Pamela, Gundogdu, Ozan, Mills, Dominic C., Inglis, Neil F., Manson, Erin, Imrie, Lisa, Bajaj-Elliott, Mona, Wren, Brendan W., Smith, David G. E., Dorrell, Nick |
| المصدر: | Infection and Immunity; October 2012, Vol. 80 Issue: 12 p4089-4098, 10p |
| مستخلص: | ABSTRACTCampylobacter jejuniis the most prevalent cause of food-borne gastroenteritis in the developed world; however, the molecular basis of pathogenesis is unclear. Secretion of virulence factors is a key mechanism by which enteric bacterial pathogens interact with host cells to enhance survival and/or damage the host. However, C. jejunilacks the virulence-associated secretion systems possessed by other enteric pathogens. Many bacterial pathogens utilize outer membrane vesicles (OMVs) for delivery of virulence factors into host cells. In the absence of prototypical virulence-associated secretion systems, OMVs could be an important alternative for the coordinated delivery of C. jejuniproteins into host cells. Proteomic analysis of C. jejuni11168H OMVs identified 151 proteins, including periplasmic and outer membrane-associated proteins, but also many determinants known to be important in survival and pathogenesis, including the cytolethal distending toxin (CDT). C. jejuniOMVs contained 16 N-linked glycoproteins, indicating a delivery mechanism by which these periplasm-located yet immunogenic glycoproteins can interact with host cells. C. jejuniOMVs possess cytotoxic activity and induce a host immune response from T84 intestinal epithelial cells (IECs), which was not reduced by OMV pretreatment with proteinase K or polymyxin B prior to coincubation with IECs. Pretreatment of IECs with methyl-beta-cyclodextrin partially blocks OMV-induced host immune responses, indicating a role for lipid rafts in host cell plasma membranes during interactions with C. jejuniOMVs. OMVs isolated from a C. jejuni11168H cdtAmutant induced interleukin-8 (IL-8) to the same extent as did wild-type OMVs, suggesting OMV induction of IL-8 is independent of CDT. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00199567 10985522 |
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| DOI: | 10.1128/IAI.00161-12 |