المؤلفون: Chandrayee Deshamukhya, Deepshikha Bhowmik, Debadatta Dhar (Chanda), Amitabha Bhattacharjee

المصدر: Indian Journal of Medical Research, Vol 157, Iss 5, Pp 477-481 (2023)

مصطلحات موضوعية: Medicine

العلاقة: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2023;volume=157;issue=5;spage=477;epage=481;aulast=DeshamukhyaTest; https://doaj.org/toc/0971-5916Test; https://doaj.org/article/c2e27d43b0e24ff5b23a128c7819dcfbTest

الإتاحة: https://doi.org/10.4103/ijmr.ijmr_3304_21Test
https://doaj.org/article/c2e27d43b0e24ff5b23a128c7819dcfbTest

المؤلفون: Birson Ingti, Deepika B Krishnatreya, Anand Prakash Maurya, Debadatta Dhar (Chanda), Atanu Chakravarty, Amitabha Bhattacharjee

المصدر: Indian Journal of Medical Research, Vol 145, Iss 5, Pp 659-664 (2017)

مصطلحات موضوعية: AmpC β-lactamase - blaPDC- Pseudomonas aeruginosa, Medicine

الوصف: Background & objectives: Pseudomonas aeruginosa possessing chromosomally inducible blaPDCalong with other intrinsic mechanism causes infection with high mortality rate. It is difficult to detect inducible AmpC enzymes in this organism and is usually overlooked by routine testing that may lead to therapeutic failure. Therefore, three different inducers were evaluated in the present study to assess their ability of induction of blaPDCin P. aeruginosa. Methods: A total of 189 consecutive Pseudomonas isolates recovered from different clinical specimens (November 2011-April 2013) were selected for the study. Isolates were screened with cefoxitin for AmpC β-lactamases and confirmed by modified three-dimensional extract test (M3DET). Inductions were checked using three inducers, namely, clavulanic acid, cefoxitin and imipenem along with ceftazidime. Molecular screening of AmpC β-lactamase genes was performed by PCR assay. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) were determined, and repetitive extragenic palindromic-PCR of all blaPDCharbouring isolates was performed. Results: Inducible phenotype was observed in 42 (24.3%) of 97 (56%) isolates confirmed by M3DET. Among these, 22 isolates harboured chromosomal blaPDCgene, and cocarriage of both chromosomal and plasmid-mediated blaAmpC genes was observed in seven isolates. Cefoxitin-ceftazidime-based test gave good sensitivity and specificity for detecting inducible AmpC enzymes. Isolates harbouring blaPDCshowed high MIC against all tested cephalosporins and monobactam. DNA fingerprinting of these isolates showed 22 different clones of P. aeruginosa. Interpretation & conclusions: P. aeruginosa harbouring inducible (chromosomal) and plasmid-mediated AmpC β-lactamase is a matter of concern as it may limit therapeutic option. Using cefoxitin-ceftazidime-based test is simple and may be used for detecting inducible AmpC β-lactamase amongst P. aeruginosa.

العلاقة: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2017;volume=145;issue=5;spage=659;epage=664;aulast=IngtiTest; https://doaj.org/toc/0971-5916Test; https://doaj.org/article/00e3770e49ec46788a3e77922cd608b1Test

الإتاحة: https://doi.org/10.4103/ijmr.IJMR_628_15Test
https://doaj.org/article/00e3770e49ec46788a3e77922cd608b1Test

المؤلفون: Deepjyoti Paul, Anand Prakash Maurya, Debadatta Dhar Chanda, Gauri Dutt Sharma, Atanu Chakravarty, Amitabha Bhattacharjee

المصدر: Indian Journal of Medical Research, Vol 143, Iss 6, Pp 826-829 (2016)

مصطلحات موضوعية: Medicine

العلاقة: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2016;volume=143;issue=6;spage=826;epage=829;aulast=PaulTest; https://doaj.org/toc/0971-5916Test; https://doaj.org/article/2a6c34a305d74878882bf80cebbbf276Test

الإتاحة: https://doi.org/10.4103/0971-5916.192079Test
https://doaj.org/article/2a6c34a305d74878882bf80cebbbf276Test

المؤلفون: Anand Prakash Maurya, Debarati Choudhury, Anupam Das Talukdar, Debadatta Dhar(Chanda), Atanu Chakravarty, Amitabha Bhattacharjee

المصدر: Indian Journal of Medical Research, Vol 141, Iss 6, Pp 816-822 (2015)

مصطلحات موضوعية: ESBLs - gene cassette - Gram-negative - Inc group - integron - PER - plasmids, Medicine

الوصف: Background & objectives: Pseudomonas extended resistant (PER) enzymes are rare type of extended-spectrum beta lactamases (ESBLs) that confer third generation cephalosporin resistance. These are often integron borne and laterally transmitted. The aim of the present study was to investigate the emergence of integron borne cephalosporin resistant PER-1 gene in diverse incompatibility (Inc) group plasmids among Gram-negative bacteria. Methods: A total of 613 consecutive, non-duplicate, Gram-negative bacteria of Enterobacteriaceae family and non-fermenting Gram-negative bacteria were isolated from different clinical specimens during a period of 18 months. For amplification and detection of blaPER, multiplex PCR was done. For understanding the genetic environment of blaPER-1, integrase gene PCR and cassette PCR (59 be) was performed. Gene transferability experiment was carried out and PCR based replicon typing was performed for incompatibility group typing of plasmids using 18 pairs of primers. An inhibitor based method was used for phenotypic detection of intrinsic resistance. Results: Multiplex PCR and sequencing confirmed that 45 isolates were harbouring blaPER-1. Both class 1 and class 2 integrons were observed among them. Integrase and cassette PCR (59 be) PCR results confirmed that the resistant determinant was located within class 1 integron. Transformation and conjugation experiments revealed that PER-1 was laterally transferable and disseminated through diverse Inc plasmid type. Efflux pump mediated carbapenem resistance was observed in all isolates. All isolates belonged to heterogenous groups. Interpretation & conclusions: This study demonstrates the dissemination of cephalosporins resistant, integron borne blaPER-1 in hospital setting in this part of the country and emphasizes on the rational use of third generation cephalosporins to slow down the expansion of this rare type of ESBL gene.

العلاقة: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=141;issue=6;spage=816;epage=822;aulast=MauryaTest; https://doaj.org/toc/0971-5916Test; https://doaj.org/article/920d51ffecf74928ad0a9e06f16d9521Test

الإتاحة: https://doi.org/10.4103/0971-5916.160718Test
https://doaj.org/article/920d51ffecf74928ad0a9e06f16d9521Test

المؤلفون: Anand Prakash Maurya, Anupam Das Talukdar, Debadatta Dhar (Chanda), Atanu Chakravarty, Amitabha Bhattacharjee

المصدر: Indian Journal of Medical Research, Vol 141, Iss 3, Pp 368-369 (2015)

مصطلحات موضوعية: Medicine

العلاقة: http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=141;issue=3;spage=368;epage=369;aulast=MauryaTest; https://doaj.org/toc/0971-5916Test; https://doaj.org/article/6848301ecb044abfb3ac7e81e6726ac7Test

الإتاحة: https://doi.org/10.4103/0971-5916.156584Test
https://doaj.org/article/6848301ecb044abfb3ac7e81e6726ac7Test

المؤلفون: Debadatta Dhar, Anand Prakash Maurya, Amitabha Bhattacharjee, Atanu Chakravarty, Anupam Das Talukdar

المصدر: Indian Journal of Medical Research, Vol 141, Iss 3, Pp 368-369 (2015)
Europe PubMed Central
The Indian Journal of Medical Research
Scopus-Elsevier

مصطلحات موضوعية: Adult, Male, Imipenem, Cefotaxime, Adolescent, medicine.medical_treatment, India, lcsh:Medicine, Minocycline, Biology, medicine.disease_cause, Integron, Tigecycline, beta-Lactamases, General Biochemistry, Genetics and Molecular Biology, Microbiology, Tertiary Care Centers, Plasmid, Clavulanic acid, Correspondence, Escherichia coli, medicine, Humans, Typing, Child, Aged, lcsh:R, Bacterial Infections, General Medicine, Middle Aged, Child, Preschool, Pseudomonas aeruginosa, Beta-lactamase, biology.protein, Female, medicine.drug

الوصف: Sir, OXA-2 type beta lactamses belong to Ambler molecular class D and functional Group 2d. These types of beta lactamases are characterized by their high hydrolytic spectrum of activity against cloxacillin and oxacillin, and are poorly inhibited by clavulanic acid. Presence of this gene wasfirst reported in Pseudomonas in France1, in Escherichia coli from Israel2, and in India it was reported in E. coli in 20073. However, there is no knowledge regarding genetic environment and gene location of this resistant determinant from this part of the world. Our study reports presence of blaOXA-2 within IncF plasmid in a tertiary referral hospital of north-east India. This study was conducted in the department of Microbiology, Assam University, Silchar. A total number of 476 consecutive, non-duplicates, Gram-negative rods consisting of members of Enterobacteriaceae family and non-fermenting Gram-negative rods were isolated from different clinical specimens spanning a period of 12 months (March 2012 to February 2013) from different Wards/Clinics of Silchar Medical College and Hospital, Assam, India (Table). Screening and confirmation for extended spectrum beta lactamases (ESBLs) was done as per Clinical Laboratory Standards Institute (CLSI) guidelines4. Multiplex PCR was performed to characterize ESBL genes1. Reactions were run under the following conditions: initial denaturation 94°C for 5 min, 33 cycles of 94 °C for 35 sec, 51°C for one min, 72°C for one min and final extension at 72°C for seven min. PCR product was purified (Gene Jet Purification kit, Lithuania) and sequencing was done. For detection of class 1 and class 2 integron, integrase gene PCR was performed5. Two PCR reactions were carried out, one with HS287 and blaOXA-2 reverse, another with HS286 and blaOXA-2 forward1,6. The amplified products were further sequenced. Plasmids were purified by Gene Jet plasmid Miniprep kit (Thermo scientific, Lithuania). Transformation was carried out using Escherichia coli JM107 as recipient. Transformants were selected on cefotaxime (0.5 mg/l) containing Luria-Bertani agar (Hi-Media, Mumbai, India) plates. Conjugation experiments were carried out between clinical isolates as donors and a streptomycin resistant E. coli recipient strain B (Genei, Bangalore), transconjugants were selected on cefotaxime (0.5 mg/l) and streptomycin (800 mg/l) agar plates. For plasmid profiling, 1.5 μl of each sample was used and analyzed by agarose gel electrophoresis (1% agarose, Hi-Media, Mumbai, India), gel was run at 40V for 8 h at 18°C. PCR based replicon typing was carried out targeting 18 different replicon types, to perform five multiplex and three simplex PCRs as described previously7. Antimicrobial susceptibility was determined by Kirby Bauer disc diffusion and minimum inhibitory concentration (MIC) method4. Typing of isolates was done by enterobacterial repetitive intergenic consensus (ERIC) PCR8. Table Details of blaOXA-2 harbouring isolates A total of 15 isolates were harbouring OXA-2 gene which was further confirmed by sequencing. Co-existence of other ESBL genes was also noticed in all 15 isolates (Table). Class 1 integron was found in 13 isolates whereas one isolate carried class 2 integron and the remaining isolate carried class 1 and 2 both (Table). Sequencing results confimed that blaOXA-2 was found to be located within class I integron in 14 isolates while presence of this gene in class2 integron could not be established. Transformation results disclosed that in 13 isolates blaOXA-2 was located within the 20 kb plasmid which was also conjugatively transferable in E. coli strain B. Incompatibility typing of plasmids demonstrated that diverse Inc group types namely I1/Iγ, FIA, FIB, FIC, Y, FrepB, K and B/o were present in all blaOXA-2 harbouring isolates. But plasmid IncF was found to be common in all isolates as well as in their transformants and transconjugants. Tigecycline (n= 13; 86.66%) was the most effective antibiotics followed by imipenem (n=12; 80%) and meropenem (n=12; 80%). High MICs was observed against different groups of cephalosporins (≥256 μg/ml; n =15) and monobactam (≥256 μg/ml; n=15). All the OXA-2 producing isolates were clonally unrelated. This study indicates propagation of the blaOXA-2 by horizontal gene transfer additionally facilitated by integron mediated gene capture mechanism. Presence of this rare type of ESBL gene in diverse group of organisms and its carriage in integrons may restrict therapeutic options.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f249d0b111adfb90950dbc596ebe9f6dTest
http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2015;volume=141;issue=3;spage=368;epage=369;aulast=MauryaTest

المؤلفون: Debarati Choudhury, Anupam Das Talukdar, Chanda Debadatta Dhar, Amitabha Bhattacharjee, Anand Prakash Maurya, Atanu Chakravarty

المصدر: Indian Journal of Medical Research, Vol 141, Iss 6, Pp 816-822 (2015)
The Indian Journal of Medical Research

مصطلحات موضوعية: Adult, Male, plasmids, integron, Adolescent, medicine.drug_class, Cephalosporin, India, lcsh:Medicine, Inc group, Drug resistance, gene cassette, Integron, beta-Lactamases, General Biochemistry, Genetics and Molecular Biology, Integrons, Microbiology, Plasmid, Bacterial Proteins, Drug Resistance, Bacterial, ESBLs - gene cassette - Gram-negative - Inc group - integron - PER - plasmids, Multiplex polymerase chain reaction, Escherichia coli, medicine, Humans, Typing, Child, Aged, Cephalosporin Resistance, biology, lcsh:R, Infant, General Medicine, Middle Aged, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Gram-negative, Cephalosporins, PER, Gene cassette, ESBLs, Child, Preschool, Pseudomonas aeruginosa, biology.protein, Original Article, Female

الوصف: Background & objectives: Pseudomonas extended resistant (PER) enzymes are rare type of extended-spectrum beta lactamases (ESBLs) that confer third generation cephalosporin resistance. These are often integron borne and laterally transmitted. The aim of the present study was to investigate the emergence of integron borne cephalosporin resistant PER-1 gene in diverse incompatibility (Inc) group plasmids among Gram-negative bacteria. Methods: A total of 613 consecutive, non-duplicate, Gram-negative bacteria of Enterobacteriaceae family and non-fermenting Gram-negative bacteria were isolated from different clinical specimens during a period of 18 months. For amplification and detection of blaPER, multiplex PCR was done. For understanding the genetic environment of blaPER-1, integrase gene PCR and cassette PCR (59 be) was performed. Gene transferability experiment was carried out and PCR based replicon typing was performed for incompatibility group typing of plasmids using 18 pairs of primers. An inhibitor based method was used for phenotypic detection of intrinsic resistance. Results: Multiplex PCR and sequencing confirmed that 45 isolates were harbouring blaPER-1. Both class 1 and class 2 integrons were observed among them. Integrase and cassette PCR (59 be) PCR results confirmed that the resistant determinant was located within class 1 integron. Transformation and conjugation experiments revealed that PER-1 was laterally transferable and disseminated through diverse Inc plasmid type. Efflux pump mediated carbapenem resistance was observed in all isolates. All isolates belonged to heterogenous groups. Interpretation & conclusions: This study demonstrates the dissemination of cephalosporins resistant, integron borne blaPER-1 in hospital setting in this part of the country and emphasizes on the rational use of third generation cephalosporins to slow down the expansion of this rare type of ESBL gene.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b443b619e7df63e9d0a62be7dc9700b0Test
https://doi.org/10.4103/0971-5916.160718Test

المؤلفون: Deshamukhya, Chandrayee¹, Bhowmik, Deepshikha¹, (Chanda), Debadatta Dhar², Bhattacharjee, Amitabha¹ ab0404@gmail.com

المصدر: Indian Journal of Medical Research. May2023, Vol. 157 Issue 5, p477-481. 8p.

مصطلحات موضوعية: *STAPHYLOCOCCUS aureus

المؤلفون: Paul, Deepjyoti¹, Maurya, Anand Prakash¹, Chanda, Debadatta Dhar², Sharma, Gauri Dutt³, Chakravarty, Atanu², Bhattacharjee, Amitabha¹ ab0404@gmail.com

المصدر: Indian Journal of Medical Research. Jun2016, Vol. 143 Issue 6, p826-829. 4p.

مصطلحات موضوعية: *PSEUDOMONAS aeruginosa, *INFECTIOUS disease transmission

مستخلص: A letter to the editor is presented on a study to characterize blaNDM-1 in clinical isolates of Pseudomonas aeruginosa and their transmission dynamics.

المؤلفون: Maurya, Anand Prakash¹, Talukdar, Anupam Das², (Chanda), Debadatta Dhar³, Chakravarty, Atanu³, Bhattacharjee, Amitabha¹ ab0404@gmail.com

المصدر: Indian Journal of Medical Research. Mar2015, Vol. 141 Issue 3, p368-369. 2p.

مصطلحات موضوعية: *BETA lactamase genetics, *GRAM-negative bacteria, *HOSPITALS

مصطلحات جغرافية: INDIA

مستخلص: A letter to the editor is presented in response to a study about the presence of OXA-2 beta-lactamase producing Gram-negative bacilli in a tertiary hospital of north-east India.