دورية أكاديمية
ALDH1 & CD133 in invasive cervical carcinoma & their association with the outcome of chemoradiation therapy
| العنوان: | ALDH1 & CD133 in invasive cervical carcinoma & their association with the outcome of chemoradiation therapy |
|---|---|
| المؤلفون: | Shifa Javed, Swati Sood, Bhavana Rai, Shalmoli Bhattacharyya, Rashmi Bagga, Radhika Srinivasan |
| المصدر: | Indian Journal of Medical Research, Vol 154, Iss 2, Pp 367-374 (2021) |
| بيانات النشر: | Wolters Kluwer Medknow Publications |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | aldh1 - cancer stem cells - cd133 - cervical cancer - e-cadherin - epithelial–mesenchymal transition - keratin 7, Medicine |
| الوصف: | Background & objectives: Chemoradiation is the standard therapy for locally advanced invasive cervical cancer and response to treatment determines the outcome. Cancer stem cells (CSCs) and epithelial–mesenchymal transition (EMT) play a role in response to treatment and hence the aim of this study was to evaluate if their levels in pre-treatment biopsies by immunohistochemistry (IHC) could predict response to treatment and outcome. Methods: The study comprised 60 patients with FIGO Stage IIB/III invasive cervical carcinoma treated by chemoradiation. They were divided into two groups based on their clinical outcome: group 1, 30 patients who had no evidence of disease at 48 month follow up and group 2, 30 patients who had disease relapse within 6-12 months of treatment completion. IHC was performed for CSC markers (ALDH1, CD133, Nanog and Oct-4), EMT markers (E-cadherin and vimentin) and squamocolumnar junction (KRT7) markers and H-scores determined. Intergroup comparison was performed. The expression of these markers was also evaluated in histological sections of cervical pre-cancer (CIN1 and CIN3) in comparison to normal cervix. Results: Cervical Intraepithelial Neoplasia grade 3 (CIN3) showed high expression of ALDH1 and KRT7 as compared to normal cervical epithelium. Aldehyde dehydrogenase 1 (ALDH1) and CD133 were overexpressed in 70 and 24 per cent cervical carcinoma cases whereas E-cadherin showed reduced expression in invasive carcinoma as compared to normal controls. ALDH1 overexpression was significantly associated with disease relapse in invasive cervical carcinoma treated by chemoradiation (P<0.01). Interpretation & conclusions: Determination of ALDH1 levels in pre-treatment cervical biopsies of invasive cervical carcinoma may be useful for prediction of response to chemoradiation, with high levels predicting for a poor response. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0971-5916 |
| العلاقة: | http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2021;volume=154;issue=2;spage=367;epage=374;aulast=JavedTest; https://doaj.org/toc/0971-5916Test; https://doaj.org/article/4da5e85523b3422a9d44b86d5434aee0Test |
| DOI: | 10.4103/ijmr.IJMR_709_20 |
| الإتاحة: | https://doi.org/10.4103/ijmr.IJMR_709_20Test https://doaj.org/article/4da5e85523b3422a9d44b86d5434aee0Test |
| رقم الانضمام: | edsbas.FF1515F0 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 09715916 |
|---|---|
| DOI: | 10.4103/ijmr.IJMR_709_20 |