Contribution of Glucosylceramide Synthase to the Proliferation of Mouse Osteoblasts
| العنوان: | Contribution of Glucosylceramide Synthase to the Proliferation of Mouse Osteoblasts |
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| المؤلفون: | Yoshitaka Mishima, Koichi Furukawa, Kazunori Hamamura, Tetsuya Okajima, Hanami Kato, Yuko Tashima, Shigemi Goto, Akifumi Togari, Hisataka Kondo, Ken Miyazawa, Takuma Sato |
| المصدر: | In Vivo |
| بيانات النشر: | Anticancer Research USA Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cancer Research, General Biochemistry, Genetics and Molecular Biology, Bone remodeling, Mice, RNA interference, medicine, Animals, Gene silencing, Angiopoietin-Like Protein 6, Eye Proteins, Gene, Cell Proliferation, Pharmacology, Osteoblasts, biology, Cell growth, Microarray analysis techniques, Chemistry, Intracellular Signaling Peptides and Proteins, Cell Differentiation, Osteoblast, Cell biology, Angiopoietin-like Proteins, medicine.anatomical_structure, Glucosyltransferases, biology.protein, ACTA2, Research Article |
| الوصف: | Background/Aim: Glycosphingolipids are known to be involved in bone metabolism. However, their roles and regulatory mechanisms in osteoblast proliferation are largely unknown. In this study, we examined the effects of inhibitors of glucosylceramide synthase (GCS), which is responsible for the generation of all glycosphingolipids, on osteoblast proliferation. Materials and Methods: We analyzed the expression of glycosphingolipids and cell growth in MC3T3-E1 mouse osteoblast cells treated with the GCS inhibitors miglustat, D-PDMP and D-PPMP. We also conducted microarray analysis and RNA interference to identify genes involved in cell growth regulated by GCS. Results: Glycosphingolipids GD1a and Gb4 expressed in MC3T3-E1 cells, were suppressed by GCS inhibitors. Furthermore, the proliferation of MC3T3-E1 cells was suppressed by the inhibitors. Using microarray analysis, we predicted nine genes (Fndc1, Acta2, Igfbp5, Cox6a2, Cth, Mymk, Angptl6, Mab21l2, and Igsf10) suppressed by all three inhibitors. Furthermore, partial silencing of Angptl6 by RNA interference reduced MC3T3-E1 cell growth. Conclusion: These results show that GCS regulates proliferation through Angptl6 in osteoblasts. |
| تدمد: | 1791-7549 0258-851X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682edf2945796d4756b4562089155837Test https://doi.org/10.21873/invivo.12606Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....682edf2945796d4756b4562089155837 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917549 0258851X |
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