دورية
Long-term organ culture of mouse mammary gland
| العنوان: | Long-term organ culture of mouse mammary gland |
|---|---|
| المؤلفون: | Harbell, J., Bowman, P., Shannon, J., Daniel, C. |
| المصدر: | In Vitro Cellular & Developmental Biology Plant; August 1977, Vol. 13 Issue: 8 p490-496, 7p |
| مستخلص: | Summary: A method for maintaining mouse mammary gland in organ culture for periods of at least 30 days is described. Strips of the number four mammary glands were cultured in individual tubes while fully submerged in Medium 199 supplemented with insulin, aldosterone, ovine prolactin and bovine growth hormone. Exchange processes were aided by slowly rotating the tubes during culture. Mammary tissue from midpregnant BALB/c and virgin GR/A mice was induced to undergo lobulo-alveolar development, secrete and remain differentiated and metabolically active for the period of culture. Cells of both the ductal and alveolar epithelium continued to synthesize DNA and divide. The submerged roller-tube culture allows the use of larger pieces of tissue than can be accommodated in static culture, and the technique may prove applicable to the culture of a variety of tissues. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 10545476 14752689 |
|---|---|
| DOI: | 10.1007/BF02615141 |