Efficient in vitro germination and shoot proliferation of chilling-treated water chestnut (Trapa japonica Flerov) embryonal explants
| العنوان: | Efficient in vitro germination and shoot proliferation of chilling-treated water chestnut (Trapa japonica Flerov) embryonal explants |
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| المؤلفون: | S. M. Rahman, A. Hoque, Susumu Arima, Yutaka Takagi |
| المصدر: | In Vitro Cellular & Developmental Biology - Plant. 37:369-374 |
| بيانات النشر: | Springer Science and Business Media LLC, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | 1-Naphthaleneacetic acid, fungi, food and beverages, Plant Science, Biology, chemistry.chemical_compound, Murashige and Skoog medium, chemistry, Shoot, Botany, Cytokinin, Kinetin, Zeatin, Gibberellic acid, Biotechnology, Explant culture |
| الوصف: | Embryonal explants from water chestnut (Trapa japonica Flerov) seeds germinated with high efficiency following a 40-d cold treatment at 5°C on half-strength MS (Murashige and Skoog) medium supplemented with 2.7 μM N6-benzyladenine (BA), 0.5 μM 1-naphthaleneacetic acid (NAA) and 0.5 μM gibberellic acid (GA3). Control and chill-treated (different durations) embryonal explants were cultured onto media which contained half-strength MS medium supplemented with different concentrations and combinations of cytokinins [BA, thidiazuron (TDZ), kinetin, zeatin], auxin (NAA) and GA3. A liquid half-strength MS medium with 1.1 μM BA and 0.5 μM NAA resulted in the best shoot proliferation of control or chill-treated explants, and the addition of 0.5 μM GA3 stimulated axillary shoot elongation. Germination and shoot proliferation were always greater for chill-treated explants compared with control explants under the same culture conditions. Shoots produced in vitro rooted 100% of the time in a liquid half-strength MS medium with 1.1 μM BA, 0.5 μM NAA and 1.1 μM indole-3-butyric acid, and the regenerated plantlets were established successfully in a water chestnut paddy field. |
| تدمد: | 1475-2689 1054-5476 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::e6c209afd96d8ff0e88ed5c95060d8a0Test https://doi.org/10.1007/s11627-001-0065-3Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........e6c209afd96d8ff0e88ed5c95060d8a0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752689 10545476 |
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