التفاصيل البيبلوغرافية
| العنوان: |
Study of diverse mechanisms of cell-mediated cytotoxicity in gene-targeted mice using flow cytometric cytotoxicity assay |
| المؤلفون: |
Malyguine, Anatoli amalyguine@mail.ncifcrf.gov, Derby, Eric1, Brooks, Alan1, Reddy, Vasavi1, Baseler, Michael1, Sayers, Thomas1 |
| المصدر: |
Immunology Letters. Aug2002, Vol. 83 Issue 1, p55. 5p. |
| مصطلحات موضوعية: |
*CELL-mediated cytotoxicity, *CANCER cells, *FLOW cytometry, *APOPTOSIS |
| مستخلص: |
Cytotoxic lymphocytes kill tumor or virus-infected target cells utilizing two mechanisms—(1) release of lytic granules (containing perforin and granzymes), and (2) Fas ligand (FasL)/Fas or TNF-initiated apoptosis. We have examined mechanisms of target cell lysis by effector T cells from gene-targeted and mutant mice using a new Flow Cytometric Cytotoxicity Assay (FC Assay). Target cells were labeled with PKH67 dye. Cell death was estimated by 7-AAD inclusion and annexin V-PE binding. A direct correlation has been found between the percentage of dead target cells in FC Assay and the results of 111In release cytotoxicity assay when effector T cells from either Pfp −/− (perforin knockout) or gld (non-functional Fas Ligand) mice were used. As shown by the 4 h FC assay, the granule-mediated mechanism was utilized by T cells from gld mice. In contrast, T cells from Pfp −/− mice used death receptor-mediated lysis. Therefore, cytotoxic cells from gene-targeted and mutant mice can serve as valuable tools for studying different mechanisms of cell-mediated cytotoxicity, and the FC assay could be applied irrespective of which cytotoxic effector pathway is involved. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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