Angiotensin II Increases Intrarenal Transforming Growth Factor-β1 in Rats Submitted to Sodium Overload Independently of Blood Pressure
| العنوان: | Angiotensin II Increases Intrarenal Transforming Growth Factor-β1 in Rats Submitted to Sodium Overload Independently of Blood Pressure |
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| المؤلفون: | Gabriel Cao, Jorge E. Toblli, Belisario E. Fernández, María Inés Rosón, Marcela Pandolfo, Silvana Della Penna, Susana Gorzalczany |
| المصدر: | Hypertension Research. 31:707-715 |
| بيانات النشر: | Japanese Society of Hypertension, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Hypertension, Renal, Physiology, Sodium, chemistry.chemical_element, Blood Pressure, Sodium Chloride, Kidney, Rats, Sprague-Dawley, Transforming Growth Factor beta1, Internal medicine, Internal Medicine, medicine, Animals, Vasoconstrictor Agents, business.industry, Angiotensin II, NF-kappa B, Actins, Rats, Disease Models, Animal, Blood pressure, Endocrinology, medicine.anatomical_structure, chemistry, Cardiology and Cardiovascular Medicine, business, Glomerular Filtration Rate, Transforming growth factor |
| الوصف: | Angiotensin II (Ang II) promotes sodium-retention, cell growth and fibrosis in addition to its classical effects on blood pressure and fluid homeostasis. In this study we examined whether low and non-hypertensive doses of exogenous Ang II could enhance the intrarenal expression of transforming growth factor-beta1 (TGF-beta1) observed in rats submitted to sodium overload. Sprague-Dawley-rats were infused for 2 h with 0.1 and 5 microg kg(-1) h(-1) Ang II (Ang 0.1 and Ang 5, respectively) together with saline solution at four different concentrations (isotonic and Na 0.5 mol L(-1), Na 1.0 mol L(-1) and Na 1.5 mol L(-1)). Renal function and mean arterial blood pressure (BP) were measured. The renal distributions of TGF-beta1, alpha-smooth-muscle-actin (alpha-SMA) and nuclear factor-kappaB (NF-kappaB) were evaluated by immunohistochemistry. While the Ang 0.1 groups were normotensive, the Ang 5 groups developed arterial hypertension progressively, and the highest blood pressure values were observed when rats were simultaneously infused with Na 1.5 mol L(-1). Glomerular function was not altered in any group. In cortical tubules, all groups infused with Ang II (0.1 and 5) and hypertonic saline solution (HSS) showed an increase in TGF-beta1 immunostaining compared to those infused with HSS alone. In medullary tubules, only the Ang 5-Na 0.5 group showed a significant increase in TGF-beta 1 immunostaining compared to the Na 0.5 group. Peritubular positive staining for alpha-SMA was present in groups receiving Ang alone or Ang-Na, in a sodium concentration-dependent manner. In cortical-tubules, NF-kappaB immunostaining was significantly increased in the Ang groups in comparison with the control and in Ang-Na 0.5 and Ang-Na 1.0 groups in comparison with the Na 0.5 mol L(-1) and Na 1.5 mol L(-1) groups, respectively, except in the case of the Ang 0.1-Na 1.5 mol L(-1) and Ang 5-Na 1.5 mol L(-1) groups. Moreover, Ang II and sodium overload induced additional changes in TGF-beta1, alpha-SMA and NF-kappaB immunostanding in glomeruli, medullary tubules and renal vessels. In conclusion, the interaction of Ang II with acute-sodium overload exacerbated intrarenal TGF-beta1, alpha-SMA and NF-kappaB expression, independently from changes in blood pressure levels, in normal rats. |
| تدمد: | 1348-4214 0916-9636 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a250738a5ff43accbb627ac17c43971bTest https://doi.org/10.1291/hypres.31.707Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a250738a5ff43accbb627ac17c43971b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13484214 09169636 |
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