Improved diagnostic stratification of digitised Barrett's oesophagus biopsies by p53 immunohistochemical staining
| العنوان: | Improved diagnostic stratification of digitised Barrett's oesophagus biopsies by p53 immunohistochemical staining |
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| المؤلفون: | Myrtle van der Wel, Jaap van der Laan, Clément J. Huysentruyt, Jacques J. Bergman, Gursah Kats-Ugurlu, Fiebo J.W. ten Kate, Mike Visser, Lodewijk A. A. Brosens, Sybren L. Meijer, G Ineke van Lijnschoten, Ariadne Ooms, Michael Doukas, Roos E. Pouw, C A Seldenrijk, Lucas C. Duits, H. Valk, Arend Karrenbeld, G. J. A. Offerhaus, Katharina Biermann, Freek Moll, Jan G.P. Tijssen |
| المساهمون: | CCA - Cancer Treatment and Quality of Life, Amsterdam Cardiovascular Sciences, Cardiology, Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology, Pathology, ACS - Heart failure & arrhythmias |
| المصدر: | Histopathology, 72(6), 1015. Wiley-Blackwell Histopathology, 1015-1023. Wiley STARTPAGE=1015;ENDPAGE=1023;ISSN=0309-0167;TITLE=Histopathology Histopathology, 72(6), 1015-1023. Wiley-Blackwell Histopathology, 72(6), 1015-1023. Wiley-Blackwell Publishing Ltd van der Wel, M J, Duits, L C, Pouw, R E, Seldenrijk, C A, Offerhaus, G J A, Visser, M, Ten Kate, F J, Biermann, K, Brosens, L A A, Doukas, M, Huysentruyt, C, Karrenbeld, A, Kats-Ugurlu, G, van der Laan, J S, van Lijnschoten, G I, Moll, F C P, Ooms, A H A G, van der Valk, H, Tijssen, J G, Bergman, J J & Meijer, S L 2018, ' Improved diagnostic stratification of digitised Barrett's oesophagus biopsies by p53 immunohistochemical staining ', Histopathology, vol. 72, no. 6, pp. 1015-1023 . https://doi.org/10.1111/his.13462Test |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | medicine.medical_specialty, Histology, Biopsy, p53 immunohistochemistry, LOW-GRADE DYSPLASIA, GUIDELINES, Pathology and Forensic Medicine, 03 medical and health sciences, Barrett Esophagus, whole-slide imaging, 0302 clinical medicine, Metaplasia, Image Interpretation, Computer-Assisted, medicine, Journal Article, MANAGEMENT, Humans, Stage (cooking), INCREASED RISK, Observer Variation, medicine.diagnostic_test, business.industry, review panel, ADENOCARCINOMA, General Medicine, Gold standard (test), medicine.disease, Immunohistochemistry, Barrett's oesophagus, NEOPLASTIC PROGRESSION, PROTEIN ACCUMULATION, Dysplasia, 030220 oncology & carcinogenesis, AGREEMENT, low-grade dysplasia, Adenocarcinoma, 030211 gastroenterology & hepatology, METAPLASIA, Radiology, interobserver agreement, OVEREXPRESSION, medicine.symptom, Tumor Suppressor Protein p53, business, Biomarkers |
| الوصف: | AIMS: Interobserver agreement for dysplasia in Barrett's oesophagus (BO) is low, and guidelines advise expert review of dysplastic cases. The aim of this study was to assess the added value of p53 immunohistochemistry (IHC) for the homogeneity within a group of dedicated gastrointestinal (GI) pathologists.METHODS AND RESULTS: Sixty-single haematoxylin and eosin (HE) slide referral BO cases [20 low-grade dysplasia (LGD); 20 high-grade dysplasia (HGD); and 20 non-dysplastic BO reference cases] were digitalised and independently assessed twice in random order by 10 dedicated GI pathologists. After a 'wash-out' period, cases were reassessed with the addition of a corresponding p53 IHC slide. Outcomes were: (i) proportion of 'indefinite for dysplasia' (IND) diagnoses; (ii) interobserver agreement; and (iii) diagnostic accuracy as compared with a consensus 'gold standard' diagnosis defined at an earlier stage by five core expert BO pathologists after their assessment of this case set. Addition of p53 IHC decreased the mean proportion of IND diagnoses from 10 of 60 to eight of 60 (P = 0.071). Mean interobserver agreement increased significantly from 0.45 to 0.57 (P = 0.0021). The mean diagnostic accuracy increased significantly from 72% to 82% (P = 0.0072) after p53 IHC addition.CONCLUSION: Addition of p53 IHC significantly improves the histological assessment of BO biopsies, even within a group of dedicated GI pathologists. It decreases the proportion of IND diagnoses, and increases interobserver agreement and diagnostic accuracy. This justifies the use of accessory p53 IHC within our upcoming national digital review panel for BO biopsy cases. |
| وصف الملف: | image/pdf; application/pdf |
| اللغة: | English |
| تدمد: | 0309-0167 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8673cc92f0790b5412ad142a3bf55fdTest https://hdl.handle.net/11370/3b5cb609-7758-4881-b734-cb9c28587276Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c8673cc92f0790b5412ad142a3bf55fd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 03090167 |
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