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1
المصدر: Histopathology. 81:108-118
مصطلحات موضوعية: Histology, Carcinosarcoma, Biomarkers, Tumor, Humans, Zinc Finger E-box-Binding Homeobox 1, Female, General Medicine, Carcinoma, Endometrioid, Immunohistochemistry, Carcinoma, Neuroendocrine, Endometrial Neoplasms, Pathology and Forensic Medicine
الوصف: The pathological diagnosis of undifferentiated and de-differentiated endometrial carcinomas (UC/DCs) is prognostically important. However, undifferentiated components may be confused with other subtypes, particularly grade 3 endometrioid carcinomas (G3ECs). Zinc finger E-box binding homeobox 1 (ZEB1) has recently been identified as a promising marker because it is frequently expressed in the undifferentiated components of UC/DCs, but not in other carcinomas. Therefore, we herein evaluated the diagnostic utility of ZEB1 with an emphasis on distinguishing between UC/DCs and G3ECs using an expanded cohort of endometrial carcinomas and carcinosarcomas.Immunostaining for ZEB1 was performed on whole-tissue sections of 19 UC/DCs, 194 non-UC/DC endometrial carcinomas and 29 carcinosarcomas. Staining was defined as negative ( 5%), focal (5-50%) and diffuse expression ( 50%). ZEB1 was expressed in 84% of the undifferentiated components of UC/DCs (diffuse in 14, focal in two). Focal expression was observed in eight non-UC/DC endometrial carcinomas and diffuse expression in seven, with the latter comprising G3ECs (four of 76), serous carcinoma (one of 37), clear cell carcinoma (one of 21) and neuroendocrine carcinoma (one of three). Epithelial differentiation was morphologically and immunohistochemically less evident in G3ECs and neuroendocrine carcinoma with diffuse ZEB1 expression. All carcinosarcomas showed diffuse ZEB1 expression in their sarcomatous components.Immunostaining for ZEB1 was sufficiently sensitive to detect undifferentiated components. Diffuse ZEB1 expression showed high specificity for distinguishing between undifferentiated components and G3ECs; however, ZEB1 expression was not entirely specific to UC/DCs. The integration of ZEB1 into the diagnosis of UC/DCs requires careful examination to exclude other tumours, such as less differentiated G3ECs, neuroendocrine carcinomas and carcinosarcomas.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e694a811c20b3b4677887c0c4cd43c89Test
https://doi.org/10.1111/his.14671Test -
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المؤلفون: Toshiro Kubota, Kimio Wakana, Atsushi Kihara, Masanobu Kitagawa
المصدر: Histopathology. 69:374-382
مصطلحات موضوعية: Adult, 0301 basic medicine, Oncology, medicine.medical_specialty, Histology, Serous carcinoma, Slug, Kaplan-Meier Estimate, Snail, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, biology.animal, Internal medicine, Biomarkers, Tumor, medicine, Adjuvant therapy, Carcinoma, Humans, Aged, Proportional Hazards Models, biology, business.industry, fungi, Hazard ratio, General Medicine, Middle Aged, Prognosis, medicine.disease, biology.organism_classification, Immunohistochemistry, Endometrial Neoplasms, 030104 developmental biology, 030220 oncology & carcinogenesis, Clear cell carcinoma, Female, Snail Family Transcription Factors, Neoplasm Grading, Neoplasm Recurrence, Local, business
الوصف: Aims To investigate how SNAIL and SLUG were involved in the nature of high-grade endometrial carcinomas (grade 3 endometrioid carcinoma, serous carcinoma and clear cell carcinoma), we analysed the correlation of their expression status with clinicopathological characteristics and evaluated their prognostic significance. Methods and results We performed immunohistochemical staining in 52 high-grade endometrial carcinomas. Expression status of SNAIL and SLUG was classified into a high expression (positive in more than 50% of the tumour cells) and a low expression. Thirteen cases (25%) showed a high expression of SLUG, whereas all 52 cases showed a low expression of SNAIL. High expression of SLUG was correlated significantly with tumour recurrence (P = 0.0203) and aberrant p53 expression (P = 0.000559). Overall survival was worse in patients with high SLUG expression at all stages (P = 0.0327) and in those who underwent adjuvant therapy (P = 0.00963). Among the patients with complete tumour resection, high SLUG expression was associated with worse recurrence-free survival (RFS) in the patients at all stages (P = 0.00264), at stages III/IV (P = 0.0146), and who underwent adjuvant therapy (P = 0.000743). SLUG expression was identified as an independent factor of RFS by multivariate analysis (hazard ratio 5.938, 95% confidence interval 1.251–28.18, P = 0.025). Conclusions SLUG expression could be correlated with TP53 mutational status and could be involved in therapeutic resistance resulting in tumour recurrence. A high expression level of SLUG can be an indicator of recurrence and a therapeutic target for long-term remission in high-grade endometrial carcinomas.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e10a742e081a9603179e57548ea5f99Test
https://doi.org/10.1111/his.12971Test
