المؤلفون: Yu Xiao, Ling Wang, Shi-xu Li, Shi-song Fang, Fan Luo, Shu-liang Chen, Xuan Zou, Lin Ye, Wei Hou

المصدر: Heliyon, Vol 10, Iss 9, Pp e30044- (2024)

مصطلحات موضوعية: COVID-19, Limbal, Cell model, Drug screening, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: To minimize the global pandemic COVID-19 spread, understanding the possible transmission routes of SARS-CoV-2 and discovery of novel antiviral drugs are necessary. We describe here that the virus can infect ocular surface limbal epithelial, but not other regions. Limbal supports wild type and mutant SARS-CoV-2 entry and replication depending on ACE2, TMPRSS2 and possibly other receptors, resulting in slight CPE and arising IL-6 secretion, which symbolizes conjunctivitis in clinical symptoms. With this limbal model, we have screened two natural product libraries and discovered several unreported drugs. Our data reveal important commonalities between COVID-19 and ocular infection with SARS-CoV-2, and establish an ideal cell model for drug screening and mechanism research.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024060754Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/ba05cd61a8eb493f9177025402593bbbTest

المؤلفون: Li-na Zhu, Deng Chen, Chengqi He

المصدر: Heliyon, Vol 10, Iss 4, Pp e26540- (2024)

مصطلحات موضوعية: PEMF, Metabolomics, PD, SH-SY5Y, Cell model, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Objective: PEMF is an emerging technique in the treatment of Parkinson's disease (PD) due to its potential improvement of movement speed. The aim of this study was to investigate the metabolic profiles of pulsed electromagnetic fields (PEMFs) in an SH-SY5Y cell model of PD. Methods: The SH-SY5Y cell model of PD was induced by 1-methyl-4-phenylpyridinium (MPP+). Liquid chromatography mass spectrometry (LC‒MS)-based untargeted metabolomics was performed to examine changes in the PD cell model with or without PEMF exposure. We conducted KEGG pathway enrichment analysis to explore the potentially related pathways of the differentially expressed metabolites. Results: A total of 275 metabolites were annotated, and 27 significantly different metabolites were found between the PEMF treatment and control groups (VIP >1, P

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024025714Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/3ade1adb09f84fae9aadd7263c2a28e3Test

المؤلفون: Clovis Ntahkie Takembo, Timoleon Crepin Kofane

المصدر: Heliyon, Vol 8, Iss 3, Pp e09084- (2022)

مصطلحات موضوعية: Myocardial cell model, Electromagnetic induction, Bursting, Mixed frequencies magnetic radiation, Bifurcation, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Based on the standard Fitzhugh–Nagumo model for myocardial cell excitations and electrical activities, the effect of electromagnetic induction is considered and through which mixed frequencies magnetic radiation is imposed to detect the mode transition. Indeed, time-varying electromagnetic field can be induced when myocardial cell is exposed or surrounded by electromagnetic field and thus the effect of electromagnetic induction should be considered. From the analyzes of sampled series for membrane potentials, the improved model holds many bifurcation parameters and the mode of excitations and electric activities can be detected and observed in larger parameter zones. It is found that apart from exciting a myocardial cell, the mixed frequencies magnetic radiation can promote mode transition to bursting type behavior as the frequency is increased as well as suppress the electrical activities to quiescent state under high intensities magnetic radiations, which are consistent with biological experiments.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844022003723Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/bff7fbd22f694d0b9b9ad46febd0f7faTest

المؤلفون: Susumu Kawakami, Takehiro Ito, Yoshinari Makino, Makoto Hashimoto, Masafumi Yano

المصدر: Heliyon, Vol 6, Iss 11, Pp e05397- (2020)

مصطلحات موضوعية: Cell model, Curvature-circle detection, 3D normal-line transform, Column, Coarse-to-fine extraction, Cell-array conversion, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: The contour of an arbitrary figure can be represented as a group of circles of curvature in contact with it, with each curvature circle represented by its center OC and radius r. We propose a series of cell models for detecting this circle, which is composed of a lateral geniculate nucleus (LGN) cell, nondirectionally selective (NDS) simple cell, and curvature-circle detection cell (CDC). The LGN and NDS simple cells were previously modeled. The CDC has been modeled as follows. Each tangent in contact with this circle is detected by an NDS simple cell that performs the Hough transformation of LGN cell responses, and then this tangent is transformed to a three-dimensional (3D) normal line in a CDC column. This transformation has been named a 3D normal-line transform. Performing this transformation for all tangents causes a CDC at the intersection of these normal lines to fire most intensively, and thus the OC and r of the circle is detected as the coordinates of this intersection. Therefore, the CDC has been modeled as this 3D normal-line transform. Based on this CDC, we model two types of constancy CDC: a position-invariant CDC and a curvature-invariant CDC. These three types of CDC reflect the response to various stimuli in actual area V4 cells. In order to validate these CDC types neurophysiologically, we propose an experimental method using microelectrodes. Cell models previously reported correspond to this hierarchy: the S1, S2, and C2 cells correspond to the NDS simple cell, CDC, and position-invariant CDC, respectively.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844020322404Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/f332b578adc04a0ba182a5e17fb0b383Test

المؤلفون: Duo Xu, Shuyan Qiu, Rong Zhou, Hong-Kai Wu, Li Zhang, Wenkuan Liu, Yun Xu

المصدر: Heliyon
Heliyon, Vol 6, Iss 6, Pp e04243-(2020)

مصطلحات موضوعية: 0301 basic medicine, Proteomics, Cell biology, Bioinformatics, Molecular biology, Cellular differentiation, Cell, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, In vivo, Human primary bronchial/tracheal epithelial cell, medicine, Cell differentiation, KEGG, lcsh:Social sciences (General), lcsh:Science (General), Multidisciplinary, Human airway epithelium, Cell cycle, Epithelium, 030104 developmental biology, medicine.anatomical_structure, Cell model, lcsh:H1-99, 030217 neurology & neurosurgery, lcsh:Q1-390

الوصف: Un-differentiated (UD) and well-differentiated (WD) normal human primary bronchial/tracheal epithelial cells are important respiratory cell models. Mature, WD cells which can be derived by culturing UD cells at an air-liquid interface represent a good surrogate for in vivo human airway epithelium. The overall protein profile of WD cells is poorly understood; therefore, the current study evaluated the proteomic characteristics of WD and UD cells using label-free LC-MS/MS and LC-PRM/MS. A total of 3,579 proteins were identified in WD and UD cells. Of these, 198 proteins were identified as differentially expressed, with 121 proteins upregulated and 77 proteins downregulated in WD cells compared with UD cells. Differentially expressed proteins were mostly enriched in categories related to epithelial structure formation, cell cycle, and immunity. Fifteen KEGG pathways and protein interaction networks were enriched and identified. The current study provides a global protein profile of WD cells, and contributes to understanding the function of human airway epithelium.
Bioinformatics; Cell biology; Molecular biology; Human airway epithelium; Human primary bronchial/tracheal epithelial cell; Cell differentiation; Proteomics; Cell model

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e209adfbcbdb0b683f92207eb4876bb1Test
http://europepmc.org/articles/PMC7322050Test

المؤلفون: Makoto Hashimoto, Yoshinari Makino, Susumu Kawakami, Takehiro Ito, Masafumi Yano

المصدر: Heliyon
Heliyon, Vol 6, Iss 11, Pp e05397-(2020)

مصطلحات موضوعية: Nervous system, 0301 basic medicine, Consciousness, Column, Simple cell, Curvature, Lateral geniculate nucleus, Hough transform, law.invention, Combinatorics, 03 medical and health sciences, Cognition, 0302 clinical medicine, Intersection, law, Curvature-circle detection, medicine, Information systems, Mathematical biosciences, lcsh:Social sciences (General), lcsh:Science (General), 3D normal-line transform, Emotion, Physics, Multidisciplinary, Behavioral neuroscience, Systems neuroscience, Tangent, Coarse-to-fine extraction, 030104 developmental biology, Transformation (function), medicine.anatomical_structure, Cell-array conversion, Cell model, lcsh:H1-99, Shape recognition, Normal, 030217 neurology & neurosurgery, lcsh:Q1-390, Research Article

الوصف: The contour of an arbitrary figure can be represented as a group of circles of curvature in contact with it, with each curvature circle represented by its center OC and radius r. We propose a series of cell models for detecting this circle, which is composed of a lateral geniculate nucleus (LGN) cell, nondirectionally selective (NDS) simple cell, and curvature-circle detection cell (CDC). The LGN and NDS simple cells were previously modeled. The CDC has been modeled as follows. Each tangent in contact with this circle is detected by an NDS simple cell that performs the Hough transformation of LGN cell responses, and then this tangent is transformed to a three-dimensional (3D) normal line in a CDC column. This transformation has been named a 3D normal-line transform. Performing this transformation for all tangents causes a CDC at the intersection of these normal lines to fire most intensively, and thus the OC and r of the circle is detected as the coordinates of this intersection. Therefore, the CDC has been modeled as this 3D normal-line transform. Based on this CDC, we model two types of constancy CDC: a position-invariant CDC and a curvature-invariant CDC. These three types of CDC reflect the response to various stimuli in actual area V4 cells. In order to validate these CDC types neurophysiologically, we propose an experimental method using microelectrodes. Cell models previously reported correspond to this hierarchy: the S1, S2, and C2 cells correspond to the NDS simple cell, CDC, and position-invariant CDC, respectively.
Cell model, Curvature-circle detection, 3D normal-line transform, Column, Coarse-to-fine extraction, Cell-array conversion, Shape recognition, Information systems, Behavioral neuroscience, Nervous system, Cognition, Consciousness, Emotion, Systems neuroscience, Mathematical biosciences.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e33cf342d4f6e5cc552ec7c456b43b70Test
https://doi.org/10.1016/jTest.heliyon.2020.e05397