Protein profile of well-differentiated versus un-differentiated human bronchial/tracheal epithelial cells
| العنوان: | Protein profile of well-differentiated versus un-differentiated human bronchial/tracheal epithelial cells |
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| المؤلفون: | Duo Xu, Shuyan Qiu, Rong Zhou, Hong-Kai Wu, Li Zhang, Wenkuan Liu, Yun Xu |
| المصدر: | Heliyon Heliyon, Vol 6, Iss 6, Pp e04243-(2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Proteomics, Cell biology, Bioinformatics, Molecular biology, Cellular differentiation, Cell, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, In vivo, Human primary bronchial/tracheal epithelial cell, medicine, Cell differentiation, KEGG, lcsh:Social sciences (General), lcsh:Science (General), Multidisciplinary, Human airway epithelium, Cell cycle, Epithelium, 030104 developmental biology, medicine.anatomical_structure, Cell model, lcsh:H1-99, 030217 neurology & neurosurgery, lcsh:Q1-390 |
| الوصف: | Un-differentiated (UD) and well-differentiated (WD) normal human primary bronchial/tracheal epithelial cells are important respiratory cell models. Mature, WD cells which can be derived by culturing UD cells at an air-liquid interface represent a good surrogate for in vivo human airway epithelium. The overall protein profile of WD cells is poorly understood; therefore, the current study evaluated the proteomic characteristics of WD and UD cells using label-free LC-MS/MS and LC-PRM/MS. A total of 3,579 proteins were identified in WD and UD cells. Of these, 198 proteins were identified as differentially expressed, with 121 proteins upregulated and 77 proteins downregulated in WD cells compared with UD cells. Differentially expressed proteins were mostly enriched in categories related to epithelial structure formation, cell cycle, and immunity. Fifteen KEGG pathways and protein interaction networks were enriched and identified. The current study provides a global protein profile of WD cells, and contributes to understanding the function of human airway epithelium. Bioinformatics; Cell biology; Molecular biology; Human airway epithelium; Human primary bronchial/tracheal epithelial cell; Cell differentiation; Proteomics; Cell model |
| اللغة: | English |
| تدمد: | 2405-8440 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e209adfbcbdb0b683f92207eb4876bb1Test http://europepmc.org/articles/PMC7322050Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e209adfbcbdb0b683f92207eb4876bb1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24058440 |
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