التفاصيل البيبلوغرافية
| العنوان: |
Resistance to apoptosis-inducing stimuli in CD44+ head and neck squamous cell carcinoma cells |
| المؤلفون: |
Atsushi Okamoto, Kazuaki Chikamatsu, Goro Takahashi, Koichi Sakakura, Keisuke Masuyama, Hiroki Ishii, Motohiro Moriyama |
| المصدر: |
Head & Neck. 34:336-343 |
| بيانات النشر: |
Wiley, 2011. |
| سنة النشر: |
2011 |
| مصطلحات موضوعية: |
Oncology, medicine.medical_specialty, Cell Culture Techniques, Apoptosis, Inhibitor of apoptosis, Flow cytometry, Cancer stem cell, Cell Line, Tumor, Internal medicine, Humans, Medicine, Cell Proliferation, biology, medicine.diagnostic_test, Squamous Cell Carcinoma of Head and Neck, business.industry, Cell Cycle, CD44, Cancer, Cell cycle, medicine.disease, Head and neck squamous-cell carcinoma, Neoplasm Proteins, Hyaluronan Receptors, Otorhinolaryngology, Head and Neck Neoplasms, Tumor Necrosis Factors, Carcinoma, Squamous Cell, Neoplastic Stem Cells, Cancer research, biology.protein, Apoptosis Regulatory Proteins, business |
| الوصف: |
Background CD44 was identified previously as a surface marker in cancer stem cells (CSCs) of head and neck squamous cell carcinoma (HNSCC). Most cancer treatments have been linked to the activation of the apoptosis-signaling pathway; however, the resistance mechanisms to apoptosis in CSCs have not yet been fully elucidated. Methods The sensitivity of CD44+ cells to diverse apoptosis-inducing stimuli was compared with that of CD44- cells. Furthermore, cell cycle changes and the expression of anti-apoptosis-related genes were examined using flow cytometry and real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results CD44+ cells were resistant to various apoptosis-inducing stimuli. Moreover, CD44+ cells showed a higher proportion of cells in G2/M phase of the cell cycle and upregulation of Bcl-2 and inhibitor of apoptosis (IAP) family genes compared with CD44- cells. Conclusion Treatment resistance in CSCs seems to be regulated by various mechanisms, and, therefore, additional treatment strategies to target CSCs are required in patients with HNSCC. © 2011 Wiley Periodicals, Inc. Head Neck, 2012 |
| تدمد: |
1043-3074 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfed19f4c7c2321ec9cbf6065ee74339Test
https://doi.org/10.1002/hed.21732Test |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi.dedup.....cfed19f4c7c2321ec9cbf6065ee74339 |
| قاعدة البيانات: |
OpenAIRE |
