التفاصيل البيبلوغرافية
| العنوان: |
Genistein Inhibits Cell Proliferation and Stimulates Apoptosis in Human Coronary Artery Endothelial Cells. |
| المؤلفون: |
Kayisli, Umit A., Guzeloglu-Kayisli, Ozlem, Guzel, Elif, Arici, Aydin |
| المصدر: |
Gynecologic & Obstetric Investigation; May2013, Vol. 75 Issue 4, p235-242, 8p, 2 Diagrams, 3 Graphs |
| مصطلحات موضوعية: |
GENISTEIN, ISOFLAVONES, ESTRADIOL, CORONARY arteries, IMMUNOCYTOCHEMISTRY, ENDOTHELIAL cells |
| مستخلص: |
Background/Aims: Isoflavone genistein is a plant-derived compound structurally similar to estradiol, which behaves weakly estrogenic or anti-estrogenic in a cell- and concentration-dependent manner. Genistein has been hypothesized to have beneficial effects on vascular diseases, although the mechanism has been unclear. Here, we investigated whether genistein may play a role in atherogenesis by regulating human coronary artery endothelial cell (HCAEC) survival. Methods: HCAECs obtained from 48- to 53-year-old women (n = 3) were used and immunocytochemistry, cell proliferation assay and apoptosis assay were carried on HCAECs treated by genistein. Results: Immunocytochemistry confirmed that HCAECs in culture express predominantly ESR2. Cell proliferation assay revealed that following 72 h of genistein treatment, HCAEC proliferation decreased in a concentration-dependent (10-10 to 10-6M) manner compared to control (p < 0.01). The anti-proliferative effect of genistein is inhibited by estradiol. Genistein (10-8M) also induced a time-dependent increase in the number of apoptotic HCAECs after 24-, 48- and 72-hourtreatments as detected by TUNEL and morphological analyses. Conclusion: These findings suggest that genistein acts as an anti-proliferative agent on HCAECs. The anti-proliferative and proapoptotic effects of genistein on vascular cells underlie the proposed anti-atherogenic and cardioprotective role of genistein. Copyright © 2013 S. Karger AG, Basel [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
