دورية أكاديمية
Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C
| العنوان: | Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C |
|---|---|
| المؤلفون: | Loy, Conor J, Sotomayor-Gonzalez, Alicia, Servellita, Venice, Nguyen, Jenny, Lenz, Joan, Bhattacharya, Sanchita, Williams, Meagan E, Cheng, Alexandre P, Bliss, Andrew, Saldhi, Prachi, Brazer, Noah, Streithorst, Jessica, Suslovic, William, Hsieh, Charlotte J, Bahar, Burak, Wood, Nathan, Foresythe, Abiodun, Gliwa, Amelia, Bhakta, Kushmita, Perez, Maria A, Hussaini, Laila, Anderson, Evan J, Chahroudi, Ann, Delaney, Meghan, Butte, Atul J, DeBiasi, Roberta L, Rostad, Christina A, De Vlaminck, Iwijn, Chiu, Charles Y |
| المصدر: | GW Authored Works |
| بيانات النشر: | Health Sciences Research Commons |
| سنة النشر: | 2023 |
| المجموعة: | George Washington University: Health Sciences Research Commons (HSRC) |
| مصطلحات موضوعية: | DNA damage, RNA sequencing, RNA-seq, SARS-CoV-2, bisulfite sequencing, cell damage, cell-free DNA, cell-free RNA, clinical severity, coronavirus disease 2019, disease biomarkers, host response, immune response, multisystem inflammatory syndrome in children, nucleic acid sequencing, pediatric, signaling pathways, systems biology, tissue damage, whole-blood RNA |
| الوصف: | Differential host responses in coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome in children (MIS-C) remain poorly characterized. Here, we use next-generation sequencing to longitudinally analyze blood samples from pediatric patients with COVID-19 or MIS-C across three hospitals. Profiling of plasma cell-free nucleic acids uncovers distinct signatures of cell injury and death between COVID-19 and MIS-C, with increased multiorgan involvement in MIS-C encompassing diverse cell types, including endothelial and neuronal cells, and an enrichment of pyroptosis-related genes. Whole-blood RNA profiling reveals upregulation of similar pro-inflammatory pathways in COVID-19 and MIS-C but also MIS-C-specific downregulation of T cell-associated pathways. Profiling of plasma cell-free RNA and whole-blood RNA in paired samples yields different but complementary signatures for each disease state. Our work provides a systems-level view of immune responses and tissue damage in COVID-19 and MIS-C and informs future development of new disease biomarkers. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://hsrc.himmelfarb.gwu.edu/gwhpubs/2706Test; https://doi.org/10.1016/j.xcrm.2023.101034Test |
| DOI: | 10.1016/j.xcrm.2023.101034 |
| الإتاحة: | https://doi.org/10.1016/j.xcrm.2023.101034Test https://hsrc.himmelfarb.gwu.edu/gwhpubs/2706Test |
| رقم الانضمام: | edsbas.53A1BC73 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.xcrm.2023.101034 |
|---|