The intestinal microbiota and metabolites in patients with anorexia nervosa
| العنوان: | The intestinal microbiota and metabolites in patients with anorexia nervosa |
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| المؤلفون: | Marek Kuzma, Martin Bilej, Alena Lambertova, Jakub Kreisinger, Petra Procházková, Jiri Dvorak, Radka Roubalová, Josef Bulant, Helena Tlaskalova-Hogenova, Helena Pelantová, Kvido Smitka, Martina Cermakova, Hana Papezova, Petra Tomášová, Martin Hill, Petra Holanova |
| المصدر: | Gut Microbes, Vol 13, Iss 1 (2021) Gut Microbes article-version (VoR) Version of Record |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Anorexia Nervosa, Physiology, microbiome, RC799-869, Gut flora, Body Mass Index, Feces, 0302 clinical medicine, Brain-Gut Axis, Longitudinal Studies, Neurotransmitter Agents, biology, Gastroenterology, dysbiosis, Diseases of the digestive system. Gastroenterology, renourishment, Infectious Diseases, Female, 030211 gastroenterology & hepatology, Research Article, Research Paper, Ruminococcaceae, neurotransmitter, Adult, Microbiology (medical), Microbiology, ede-q, Young Adult, 03 medical and health sciences, mycobiome, scfa, medicine, Humans, Microbiome, bacteriome, Alistipes, gut-brain-microbiota axis, Bacteria, Clostridiales, Fungi, Bacteriome, Fatty Acids, Volatile, biology.organism_classification, medicine.disease, Archaea, Gastrointestinal Microbiome, bmi, 030104 developmental biology, Metagenome, Bacteroides, Dysbiosis |
| الوصف: | Brain-gut microbiota interactions are intensively studied in connection with various neurological and psychiatric diseases. While anorexia nervosa (AN) pathophysiology is not entirely clear, it is presumably linked to microbiome dysbiosis. We aimed to elucidate the gut microbiota contribution in AN disease pathophysiology. We analyzed the composition and diversity of the gut microbiome of patients with AN (bacteriome and mycobiome) from stool samples before and after renourishment, and compared them to healthy controls. Further, levels of assorted neurotransmitters and short-chain fatty acids (SCFA) were analyzed in stool samples by MS and NMR, respectively. Biochemical, anthropometric, and psychometric profiles were assessed. The bacterial alpha-diversity parameter analyses revealed only increased Chao 1 index in patients with AN before the realimentation, reflecting their interindividual variation. Subsequently, core microbiota depletion signs were observed in patients with AN. Overrepresented OTUs (operation taxonomic units) in patients with AN taxonomically belonged to Alistipes, Clostridiales, Christensenellaceae, and Ruminococcaceae. Underrepresented OTUs in patients with AN were Faecalibacterium, Agathobacter, Bacteroides, Blautia, and Lachnospira. Patients exhibited greater interindividual variation in the gut bacteriome, as well as in metagenome content compared to controls, suggesting altered bacteriome functions. Patients had decreased levels of serotonin, GABA, dopamine, butyrate, and acetate in their stool samples compared to controls. Mycobiome analysis did not reveal significant differences in alpha diversity and fungal profile composition between patients with AN and healthy controls, nor any correlation of the fungal composition with the bacterial profile. Our results show the changed profile of the gut microbiome and its metabolites in patients with severe AN. Although therapeutic partial renourishment led to increased body mass index and improved psychometric parameters, SCFA, and neurotransmitter profiles, as well as microbial community compositions, did not change substantially during the hospitalization period, which can be potentially caused by only partial weight recovery. |
| اللغة: | English |
| تدمد: | 1949-0984 1949-0976 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e32ef542618df4d31c5e70158575ee3Test https://doaj.org/article/22744fe77a7d4a0ebe0d9bb3f80f2b1fTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9e32ef542618df4d31c5e70158575ee3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19490984 19490976 |
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