Astrocyte arborization enhances Ca 2+ but not cAMP signaling plasticity
| العنوان: | Astrocyte arborization enhances Ca 2+ but not |
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| المؤلفون: | Matjaž Stenovec, Dorian Dolanc, Samo Pirnat, Anemari Horvat, Mićo Božić, Nina Vardjan, Robert Zorec, Alexei Verkhratsky, Petra Tavčar |
| المصدر: | Glia, vol. 69, no. 12, pp. 2899-2916, 2021. Pirnat, S, Božić, M, Dolanc, D, Horvat, A, Tavčar, P, Vardjan, N, Verkhratsky, A, Zorec, R & Stenovec, M 2021, ' Astrocyte arborization enhances Ca2+ but not cAMP signaling plasticity ', GLIA . https://doi.org/10.1002/glia.24076Test |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | vesicles, Vesicle, Cell, Central nervous system, astrocytes, Ca$^{2+}$, konfokalna mikroskopija, Biology, confocal microscopy, electrophysiology, Cell biology, Cellular and Molecular Neuroscience, Cytosol, vezikli, medicine.anatomical_structure, Neurology, In vivo, cAMP, udc:616-092, medicine, Viability assay, Homeostasis, astrociti, Astrocyte |
| الوصف: | The plasticity of astrocytes is fundamental for their principal function, maintaining homeostasis of the central nervous system throughout life, and is associated with diverse exposomal challenges. Here, we used cultured astrocytes to investigate at subcellular level basic cell processes under controlled environmental conditions. We compared astroglial functional and signaling plasticity in standard serum-containing growth medium, a condition mimicking pathologic conditions, and in medium without serum, favoring the acquisition of arborized morphology. Using opto−/electrophysiologic techniques, we examined cell viability, expression of astroglial markers, vesicle dynamics, and cytosolic Ca$^{2+}$ and cAMP signaling. The results revealed altered vesicle dynamics in arborized astrocytes that was associated with increased resting [Ca$^{2+}$]$_i$ and increased subcellular heterogeneity in [Ca$^{2+}$]$_i$, whereas [cAMP]$_i$ subcellular dynamics remained stable in both cultures, indicating that cAMP signaling is less prone to plastic remodeling than Ca$^{2+}$ signaling, possibly also in in vivo contexts. |
| وصف الملف: | application/pdf; text/url |
| تدمد: | 1098-1136 0894-1491 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dca2a647166e6f108767cdc8431b5d60Test https://doi.org/10.1002/glia.24076Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dca2a647166e6f108767cdc8431b5d60 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10981136 08941491 |
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