دورية أكاديمية
SUPPA2: fast, accurate, and uncertainty-aware differential splicing analysis across multiple conditions
| العنوان: | SUPPA2: fast, accurate, and uncertainty-aware differential splicing analysis across multiple conditions |
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| المؤلفون: | Juan L. Trincado, Juan C. Entizne, Gerald Hysenaj, Babita Singh, Miha Skalic, David J. Elliott, Eduardo Eyras |
| المصدر: | Genome Biology, Vol 19, Iss 1, Pp 1-11 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | Differential splicing, Alternative splicing, RNA-seq, Uncertainty, Biological variability, Differentiation, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract Despite the many approaches to study differential splicing from RNA-seq, many challenges remain unsolved, including computing capacity and sequencing depth requirements. Here we present SUPPA2, a new method that addresses these challenges, and enables streamlined analysis across multiple conditions taking into account biological variability. Using experimental and simulated data, we show that SUPPA2 achieves higher accuracy compared to other methods, especially at low sequencing depth and short read length. We use SUPPA2 to identify novel Transformer2-regulated exons, novel microexons induced during differentiation of bipolar neurons, and novel intron retention events during erythroblast differentiation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| العلاقة: | http://link.springer.com/article/10.1186/s13059-018-1417-1Test; https://doaj.org/toc/1474-760XTest |
| DOI: | 10.1186/s13059-018-1417-1 |
| الوصول الحر: | https://doaj.org/article/4a163ba1d9844d42876727b45744d111Test |
| رقم الانضمام: | edsdoj.4a163ba1d9844d42876727b45744d111 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-018-1417-1 |