Differential regulation of interleukin-10 production by genetic and environmental factors – a twin study
| العنوان: | Differential regulation of interleukin-10 production by genetic and environmental factors – a twin study |
|---|---|
| المؤلفون: | Esther Reuss, Christoph Becker, Anke Kruger, Thomas Höhler, Ch. Rittner, Rolf Fimmers |
| المصدر: | Genes & Immunity. 3:407-413 |
| بيانات النشر: | Springer Science and Business Media LLC, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Male, Immunology, Electrophoretic Mobility Shift Assay, Single-nucleotide polymorphism, In Vitro Techniques, Biology, Polymorphism, Single Nucleotide, Monocytes, Cell Line, Genes, Reporter, Polymorphism (computer science), Genetics, Humans, Allele, Promoter Regions, Genetic, Transcription factor, Genetics (clinical), Binding Sites, Haplotype, DNA, Heritability, Twin study, Interleukin-10, Interleukin 10, Gene Expression Regulation, Twin Studies as Topic, Female, Transcription Factors |
| الوصف: | Interleukin-10 (IL-10) has a critical role in the regulation of immune responses. The relative contribution of genetic and environmental factors to IL-10 production is under debate. We performed a twin study in 246 monozygotic and dizygotic twins to assess the heritability of IL-10 production after LPS stimulation in whole blood. In addition, the influence of promoter single nucleotide polymorphisms (-1082, -819 and -592) on transcriptional activity and their binding to nuclear factors was studied in luciferase reporter gene and electrophoretic mobility shift assays. IL-10 production showed a genetic determination with a heritability of 0.5. Decreasing body mass index (BMI), smoking and female gender lead to decreased IL-10 production. In monocytes, the -1082A allele showed higher binding affinity to the transcription factor PU.1 resulting in decreased transcriptional activity of -1082A promoter haplotypes. Genetic determination of IL-10 secretion is probably lower than that previously reported. Fifty percent of the observed variability explained by genetic factors. Female individuals produce less IL-10 than male subjects. Environmental factors like smoking and decreasing BMI exert suppressing effects on IL-10 production. Although the -1082A allele shows higher binding affinity to the PU.1 transcription factor and lower transcriptional activity, this polymorphism probably explains only a small fraction of the observed heritability. |
| تدمد: | 1476-5470 1466-4879 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c234b6c829ba87de0d233034341c841Test https://doi.org/10.1038/sj.gene.6363920Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5c234b6c829ba87de0d233034341c841 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765470 14664879 |
|---|