Identification of Long Non-Coding RNAs Involved in Porcine Fat Deposition Using Two High-Throughput Sequencing Methods
| العنوان: | Identification of Long Non-Coding RNAs Involved in Porcine Fat Deposition Using Two High-Throughput Sequencing Methods |
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| المؤلفون: | Kai Xing, Hong Ao, Ying Yu, Xitong Zhao, Yibing Liu, Yong Shi, Chuduan Wang, Fengxia Zhang, Huatao Liu |
| المصدر: | Genes, Vol 12, Iss 1374, p 1374 (2021) Genes Volume 12 Issue 9 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | pig, Quantitative Trait Loci, Sus scrofa, Adipose tissue, RNA-Seq, Computational biology, Biology, QH426-470, DNA sequencing, ACSL3, Article, lncRNA, Genetics, Animals, Gene Regulatory Networks, Animal Husbandry, Gene, Genetics (clinical), Adiposity, ACACB, RNA, Lipid Metabolism, Adipose Tissue, fat deposition, Female, RNA, Long Noncoding, RNA-seq, Function (biology), Selective Breeding |
| الوصف: | Adipose is an important body tissue in pigs, and fatty traits are critical in pig production. The function of long non-coding RNA (lncRNA) in fat deposition and metabolism has been found in previous studies. In this study, we collected the adipose tissue of six Landrace pigs with contrast backfat thickness (nhigh = 3, nlow = 3), after which we performed strand-specific RNA sequencing (RNA-seq) based on pooling and biological replicate methods. Biological replicate and pooling RNA-seq revealed 1870 and 1618 lncRNAs, respectively. Using edgeR, we determined that 1512 genes and 220 lncRNAs, 2240 genes and 127 lncRNAs were differentially expressed in biological replicate and pooling RNA-seq, respectively. After target gene prediction, we found that ACSL3 was cis-targeted by lncRNA TCONS-00052400 and could activate the conversion of long-chain fatty acids. In addition, lncRNA TCONS_00041740 cis-regulated gene ACACB regulated the rate-limiting enzyme in fatty acid oxidation. Since these genes have necessary functions in fat metabolism, the results imply that the lncRNAs detected in our study may affect backfat deposition in swine through regulation of their target genes. Our study explored the regulation of lncRNA and their target genes in porcine backfat deposition and provided new insights for further investigation of the biological functions of lncRNA. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2073-4425 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eb139786a2f9fda3963e0d1702dce816Test https://www.mdpi.com/2073-4425/12/9/1374Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....eb139786a2f9fda3963e0d1702dce816 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734425 |
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