Time Course Analysis of Genome-Wide Identification of Mutations Induced by and Genes Expressed in Response to Carbon Ion Beam Irradiation in Rice (Oryza sativa L.)
| العنوان: | Time Course Analysis of Genome-Wide Identification of Mutations Induced by and Genes Expressed in Response to Carbon Ion Beam Irradiation in Rice (Oryza sativa L.) |
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| المؤلفون: | Tao Guo, Guili Yang, Qiling Liu, Zhiqiang Chen, Libin Zhou, Hui Wang, Wenjian Li, Jian Zhang, Ziai Peng |
| المصدر: | Genes Volume 12 Issue 9 Genes, Vol 12, Iss 1391, p 1391 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | DNA repair-related genes, Time Factors, DNA Repair, DNA repair, DNA damage, Mutant, Mutagen, QH426-470, medicine.disease_cause, whole-genome sequencing (WGS), Article, Mutation Rate, Gene Expression Regulation, Plant, Exome Sequencing, Genetics, medicine, Indel, Gene, Genetics (clinical), Plant Proteins, Mutation, Mutation breeding, Whole Genome Sequencing, Chemistry, Oryza, initial DNA damage, Molecular biology, Gene Ontology, Seeds, carbon ion beam (CIB), mutation characteristics, RNA-seq, Genome, Plant, DNA Damage |
| الوصف: | Heavy-ion irradiation is a powerful mutagen and is widely used for mutation breeding. In this study, using whole-genome sequencing (WGS) and RNA sequencing (RNA-seq) techniques, we comprehensively characterized these dynamic changes caused by mutations at three time points (48, 96, and 144 h after irradiation) and the expression profiles of rice seeds irradiated with C ions at two doses. Subsequent WGS analysis revealed that more mutations were detected in response to 40 Gy carbon ion beam (CIB) irradiation than 80 Gy of CIB irradiation at the initial stage (48 h post-irradiation). In the mutants generated from both irradiation doses, single-base substitutions (SBSs) were the most frequent type of mutation induced by CIB irradiation. Among the mutations, the predominant ones were C:T and A:G transitions. CIB irradiation also induced many short InDel mutations. RNA-seq analysis at the three time points showed that the number of differentially expressed genes (DEGs) was highest at 48 h post-irradiation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the DEGs showed that the “replication and repair” pathway was enriched specifically 48 h post-irradiation. These results indicate that the DNA damage response (DDR) and the mechanism of DNA repair tend to quickly start within the initial stage (48 h) after irradiation. |
| وصف الملف: | application/pdf |
| تدمد: | 2073-4425 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4148c2353028644b654b16ea5bbd9a1cTest https://doi.org/10.3390/genes12091391Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4148c2353028644b654b16ea5bbd9a1c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20734425 |
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