Large Scale Full-Length cDNA Sequencing Reveals a Unique Genomic Landscape in a Lepidopteran Model Insect, Bombyx mori
| العنوان: | Large Scale Full-Length cDNA Sequencing Reveals a Unique Genomic Landscape in a Lepidopteran Model Insect, Bombyx mori |
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| المؤلفون: | Nobukazu Namiki, Qingyou Xia, Kazuei Mita, Yutaka Suzuki, Marian R. Goldsmith, Toru Shimada, Sumio Sugano, Haruhiko Fujiwara, Hideki Sezutsu, Akiya Jouraku, Kiyoshi Asaoka, Hiroyuki Kanamori, Susumu Katsuma, Junko Narukawa, Shun-ichi Sasanuma, Hiroaki Noda, Kiyoko Taniai, Takaaki Daimon, Mizuko Osanai-Futahashi, Kimiko Yamamoto, Shinpei Kawaoka, Tetsuro Shinoda, Kallare P. Arunkumar, Archana Tomar, Ryo Futahashi, Yoshitaka Suetsugu, Michihiko Shimomura, Keiko Kadono-Okuda, Toshiki Tamura, Masahiro Kasahara, Masahiro Ajimura, Qili Feng, Javaregowda Nagaraju, Ryusuke Niwa, Masataka G. Suzuki, Hiroshi Kataoka |
| المصدر: | Scopus-Elsevier G3: Genes|Genomes|Genetics |
| بيانات النشر: | Oxford University Press (OUP), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, DNA, Complementary, large-scale full-length cDNA collection, Sequence analysis, Molecular Sequence Data, Sequence assembly, Investigations, Biology, gene cluster, Models, Biological, Genome, Databases, Genetic, Gene cluster, Genetics, Primer walking, Animals, silkworm, Molecular Biology, Genetics (clinical), Gene Library, Expressed Sequence Tags, Expressed sequence tag, cDNA library, fungi, Bombyx mori, Chromosome Mapping, Exons, Sequence Analysis, DNA, tissue-specific genes, Bombyx, Introns, Evolutionary biology, sexual dimorphism, Multigene Family, Female, Transcriptome, Functional genomics |
| الوصف: | The establishment of a complete genomic sequence of silkworm, the model species of Lepidoptera, laid a foundation for its functional genomics. A more complete annotation of the genome will benefit functional and comparative studies and accelerate extensive industrial applications for this insect. To realize these goals, we embarked upon a large-scale full-length cDNA collection from 21 full-length cDNA libraries derived from 14 tissues of the domesticated silkworm and performed full sequencing by primer walking for 11,104 full-length cDNAs. The large average intron size was 1904 bp, resulting from a high accumulation of transposons. Using gene models predicted by GLEAN and published mRNAs, we identified 16,823 gene loci on the silkworm genome assembly. Orthology analysis of 153 species, including 11 insects, revealed that among three Lepidoptera including Monarch and Heliconius butterflies, the 403 largest silkworm-specific genes were composed mainly of protective immunity, hormone-related, and characteristic structural proteins. Analysis of testis-/ovary-specific genes revealed distinctive features of sexual dimorphism, including depletion of ovary-specific genes on the Z chromosome in contrast to an enrichment of testis-specific genes. More than 40% of genes expressed in specific tissues mapped in tissue-specific chromosomal clusters. The newly obtained FL-cDNA sequences enabled us to annotate the genome of this lepidopteran model insect more accurately, enhancing genomic and functional studies of Lepidoptera and comparative analyses with other insect orders, and yielding new insights into the evolution and organization of lepidopteran-specific genes. |
| تدمد: | 2160-1836 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8be802b8c483f711ccef1def65811ebbTest https://doi.org/10.1534/g3.113.006239Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....8be802b8c483f711ccef1def65811ebb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21601836 |
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