التفاصيل البيبلوغرافية
| العنوان: |
Long-Term Cultivation of Human Atrial Myocardium |
| المؤلفون: |
Klumm, Maximilian J., Heim, Christian, Fiegle, Dominik J., Weyand, Michael, Volk, Tilmann, Seidel, Thomas |
| المصدر: |
Frontiers in Physiology ; volume 13 ; ISSN 1664-042X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2022 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| الوصف: |
Organotypic culture of human ventricular myocardium is emerging in basic and translational cardiac research. However, few institutions have access to human ventricular tissue, whereas atrial tissue is more commonly available and important for studying atrial physiology. This study presents a method for long-term cultivation of beating human atrial myocardium. After written informed consent, tissues from the right-atrial appendage were obtained from patients with sinus rhythm undergoing open heart surgery with cardiopulmonary bypass. Trabeculae (pectinate muscles) prepared from the samples were installed into cultivation chambers at 37°C with a diastolic preload of 500 μN. After 2 days with 0.5 Hz pacing, stimulation frequency was set to 1 Hz. Contractile force was monitored continuously. Beta-adrenergic response, refractory period (RP) and maximum captured frequency (f max ) were assessed periodically. After cultivation, viability and electromechanical function were investigated, as well as the expression of several genes important for intracellular Ca 2+ cycling and electrophysiology. Tissue microstructure was analyzed by confocal microscopy. We cultivated 19 constantly beating trabeculae from 8 patient samples for 12 days and 4 trabeculae from 3 specimen for 21 days. Functional parameters were compared directly after installation (0 d) with those after 12 d in culture. Contraction force was 384 ± 69 μN at 0 d and 255 ± 90 μN at 12 d ( p = 0.8, n = 22), RP 480 ± 97 ms and 408 ± 78 ms ( p = 0.3, n = 9), f max 3.0 ± 0.5 Hz and 3.8 ± 0.5 Hz ( p = 0.18, n = 9), respectively. Application of 100 nM isoprenaline to 11 trabeculae at 7 d increased contraction force from 168 ± 35 μN to 361 ± 60 μN ( p < 0.01), f max from 6.4 ± 0.6 Hz to 8.5 ± 0.4 Hz ( p < 0.01) and lowered RP from 319 ± 22 ms to 223 ± 15 ms. CACNA1c (L-type Ca 2+ channel subunit) and GJA1 (connexin-43) mRNA expressions were not significantly altered at 12 d vs 0 d, while ATP2A (SERCA) and KCNJ4 (Kir2.3) were downregulated, and KCNJ2 ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fphys.2022.839139 |
| DOI: |
10.3389/fphys.2022.839139/full |
| الإتاحة: |
https://doi.org/10.3389/fphys.2022.839139Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.C6A01646 |
| قاعدة البيانات: |
BASE |
