التفاصيل البيبلوغرافية
| العنوان: |
Comprehensive detection of CRLF2 alterations in acute lymphoblastic leukemia: a rapid and accurate novel approach |
| المؤلفون: |
Gil, José Vicente, Miralles, Alberto, de las Heras, Sandra, Such, Esperanza, Avetisyan, Gayane, Díaz-González, Álvaro, Santiago, Marta, Fuentes, Carolina, Fernández, José María, Lloret, Pilar, Navarro, Irene, Montesinos, Pau, Llop, Marta, Barragán, Eva |
| المصدر: |
Frontiers in Molecular Biosciences ; volume 11 ; ISSN 2296-889X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2024 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| مصطلحات موضوعية: |
Biochemistry, Genetics and Molecular Biology (miscellaneous), Molecular Biology, Biochemistry |
| الوصف: |
Introduction: Acute lymphoblastic leukemia (ALL) is a prevalent childhood cancer with high cure rate, but poses a significant medical challenge in adults and relapsed patients. Philadelphia-like acute lymphoblastic leukemia (Ph-like ALL) is a high-risk subtype, with approximately half of cases characterized by CRLF2 overexpression and frequent concomitant IKZF1 deletions. Methods: To address the need for efficient, rapid, and cost-effective detection of CRLF2 alterations, we developed a novel RT-qPCR technique combining SYBR Green and highresolution melting analysis on a single plate. Results: The method successfully identified CRLF2 expression, P2RY8::CRLF2 fusions, and CRLF2 and JAK2 variants, achieving a 100% sensitivity and specificity. Application of this method across 61 samples revealed that 24.59% exhibited CRLF2 overexpression, predominantly driven by IGH::CRLF2 (73.33%). High Resolution Melting analysis unveiled concurrent CRLF2 or JAK2 variants in 8.19% of samples, as well as a dynamic nature of CRLF2 alterations during disease progression. Discussion: Overall, this approach provides an accurate identification of CRLF2 alterations, enabling improved diagnostic and facilitating therapeutic decision-making. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fmolb.2024.1362081 |
| DOI: |
10.3389/fmolb.2024.1362081/full |
| الإتاحة: |
https://doi.org/10.3389/fmolb.2024.1362081Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.5D11EBE |
| قاعدة البيانات: |
BASE |
