Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties
| العنوان: | Biofunctionalization of Silver Nanoparticles With Lactonase Leads to Altered Antimicrobial and Cytotoxic Properties |
|---|---|
| المؤلفون: | Kshitiz Gupta, Sanjay Chhibber |
| المصدر: | Frontiers in Molecular Biosciences Frontiers in Molecular Biosciences, Vol 6 (2019) |
| بيانات النشر: | Frontiers Media SA, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, silver nanoparticles, Homoserine, lactonase, Biochemistry, Genetics and Molecular Biology (miscellaneous), Biochemistry, Silver nanoparticle, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Dynamic light scattering, Lactonase, antibiofilm activity, antivirulence, Molecular Biosciences, cell cytotoxicity, Fourier transform infrared spectroscopy, lcsh:QH301-705.5, Molecular Biology, Original Research, chemistry.chemical_classification, biology, Chemistry, biology.organism_classification, Antimicrobial, 030104 developmental biology, lcsh:Biology (General), 030220 oncology & carcinogenesis, biology.protein, Bacteria, Lactone |
| الوصف: | Background: N-acylated homoserine lactone lactonase which cleave the Acyl homoserine lactone molecules produced by biofilm-forming pathogens and silver nano-particles (AgNPs), are known for their antibacterial effect against several Gram-positive and Gram-negative bacteria. In this study, AgNPs were coated with N-acylated homoserine lactonase protein (AgNPs-AiiA) isolated from Bacillus sp. ZA12. Results: The AgNPs-AiiA complex was characterized by UV-visible spectra, Dynamic light Scattering, Fourier transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscope (Fe-SEM). The synthesized nano-particles were found to be spherical in shape and had an approximate size of 22.4 nm. Treatment with AiiA coated AgNPs showed a significant reduction in exopolysaccharide production, metabolic activity, cell surface hydrophobicity of bacterial cells, and anti-biofilm activity against multidrug-resistant K. pneumoniae as compared to treatment with AiiA protein and neat AgNPs. AgNPs-AiiA complex exhibited potent antibiofilm activity at sub-optimal concentration of 14.4 μg/mL without being harmful to the macrophages and to the various tissues including kidney, liver, spleen and lungs of BALB/c mice upon intra-venous administration. Conclusion: It is concluded that at a concentration of 14.4 μg/mL, AgNPs coated with AiiA kill bacteria without harming the host tissue and provides a suitable template to design novel anti-biofilm drug to circumvent the issue of drug resistance. |
| تدمد: | 2296-889X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcfe6aebb3ef395813f6a01848e1d930Test https://doi.org/10.3389/fmolb.2019.00063Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bcfe6aebb3ef395813f6a01848e1d930 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2296889X |
|---|