مورد إلكتروني
FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum
| العنوان: | FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum |
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| المصدر: | ISSN: 1664-302X |
| بيانات النشر: | 2019 |
| تفاصيل مُضافة: | Kong, Xiangjiu Zhang, Hao Wang, Xiaoliang Lee, T.A.J., van der Waalwijk, C. Diepeningen, A.D., van Brankovics, Balázs Xu, Jin Xu, Jingsheng Chen, Wanquan Feng, Jie |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied using knock-out and complemented strains. To fluorescently label peroxisomes’ punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH- 1 and 1FgPex3 knock-out strains. The GFP and RFP transformants in the 1FgPex3 background showed a diffuse fluorescence pattern across the cytoplasm suggesting the absence of mature peroxisomes. The 1FgPex3 strain showed a minor, non-significant reduction in growth on various sugar carbon sources. In contrast, deletion of FgPex3 affected fatty acid b-oxidation in F. graminearum and significantly reduced the utilization of fatty acids. Furthermore, the 1FgPex3 mutant was sensitive to osmotic stressors as well as to cell wall-damaging agents. Reactive oxygen species (ROS) levels in the mutant had increased significantly, which may be linked to the reduced longevity of cultured strains. The mutant also showed reduced production of conidiospores, while sexual reproduction was completely impaired. The pathogenicity of 1FgPex3, especially during the process of systemic infection, was strongly reduced on both tomato and on wheat, while to production of deoxynivalenol (DON), an important factor for virulence, appeared to be unaffected. |
| مصطلحات الفهرس: | FgPex3, fungal growth, Fusarium graminearum, peroxisome, virulence, Article/Letter to editor |
| URL: | |
| الإتاحة: | Open access content. Open access content Wageningen University & Research |
| ملاحظة: | application/pdf Frontiers in Microbiology 10 (2019) ISSN: 1664-302X ISSN: 1664-302X English |
| أرقام أخرى: | NLWUP oai:library.wur.nl:wurpubs/554078 https://library.wur.nl/WebQuery/wurpubs/554078Test 10.3389/fmicb.2019.02088 1200319523 |
| المصدر المساهم: | WUR STAFF PUBNS From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1200319523 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |